Waveguide Evanescent Field Fluorescence and Scattering Microscopy: The Status Quo
Waveguide Evanescent Field Fluorescence and Scattering Microscopy: The Status Quo
复制标题
波导倏逝场荧光和散射显微镜：现状
DOI：
10.1007/978-3-319-98548-0_1
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
S. Mittler
中科院分区：
文献类型：
--
作者：
S. Mittler
在过去的几年中，发展了波导消逝场荧光（WEFF）和散射（WEFS）显微镜，它们是TIR和TIRF显微镜的替代品。这两种技术都实现了带有耦合光栅的平板波导显微镜芯片。该技术进行了描述和比较，TIR和TIRF显微镜。波导方法的优点被清楚地解决。简要的历史技术的发展和类似的活动在该领域进行了讨论。两种WEF显微镜的应用实例如下：多模波导的静态距离映射，细胞质膜的动态溶解研究和成骨细胞对胰蛋白酶的动力学响应（WEFF）;细菌灭菌以及细胞粘附和粒度研究（WEFS）。这两种方法的组合进行了讨论，发现不合适。为了大规模制作所需的光栅波导芯片，研制了一种全聚合物波导芯片。这应该允许将新的显微镜方法带给感兴趣的科学界。
In the last few years Waveguide Evanescent Field Fluorescence (WEFF) and Scattering (WEFS) microscopy were developed which are alternatives to TIR and TIRF microscopy. Both technologies implement a slab waveguide-microscopy chip with a coupling grating. The technologies are described and compared to TIR and TIRF microscopy. The advantages of the waveguide method are clearly addressed. A brief history of the technology’s development and similar activities in the field are discussed. Application examples from both WEF microscopies follow: static distance mapping with a multimode waveguide, dynamic solubilisation studies of cell plasma membranes and the kinetic response of osteoblasts to trypsin (WEFF); bacteria sterilization as well as cell adhesion and granularity studies (WEFS). The combination of both methods is discussed and found not suitable. In order to mass fabricate the necessary waveguide chips with the grating an all-polymer-waveguide chip was developed. This should allow to bring the new microscopy methods to the interested scientific community.
影响因子：
14
作者：
Storrie, Hannah;Guler, Mustafa O.;Stupp, Samuel I.
通讯作者：
Stupp, Samuel I.
影响因子：
14
作者：
Burmeister, JS;Olivier, LA;Truskey, GA
通讯作者：
Truskey, GA
整合素粘附处的膜变形。
影响因子：
2.8
作者：
Atilgan,Erdinç;Ovryn,Ben
通讯作者：
Ovryn,Ben