Control of occlusion of middle cerebral artery in perinatal and neonatal mice with magnetic force.
Control of occlusion of middle cerebral artery in perinatal and neonatal mice with magnetic force.
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DOI：
10.1186/s13041-018-0389-0
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发表时间：
2018-08-29
期刊：
影响因子：
3.6
通讯作者：
Ge WP
中科院分区：
文献类型：
--
作者：
Jia JM;Peng C;Wang Y;Zheng J;Ge WP
缺血性围产期卒中(IPS)是一种常见的疾病，可导致严重的死亡率和发病率。在这种情况下，单侧动脉脑梗塞的发生率通常被闭塞在大脑中动脉(MCA)，导致局灶性脑缺血。在成年啮齿动物中，阻断大脑中动脉是缺血性卒中研究中最常用的策略。然而，用于IPS研究的出生后0-7天(P0-7)小鼠大脑中动脉闭塞(MCAO)模型尚未完成。在这里，我们通过诱导P0和P7之间通过小鼠颞浅静脉注射的磁性颗粒(MPS)的积聚来阻断DMCA，我们称之为新生儿或围产期单纯性(用磁性颗粒诱导中风)。SIMPLE在围产期和新生小鼠的DMCA中产生永久性或暂时性的闭塞。单纯大脑中动脉永久性闭塞可导致脑梗塞和脑内神经元死亡。SIMPLE还可以用于在新生儿或围产期小鼠的大脑中可靠地产生局灶性缺血性中风。因此，SIMPLE允许在小鼠中建立IPS或局灶性缺血性中风的模型，用于进一步的机制研究，特别适用于模拟人类早产儿的短暂性局灶性脑缺血，这在这里首次在小鼠身上完成。本文的在线版本(10.1186/s13041-0180389-0)包含补充材料，可供授权用户使用。
Ischemic perinatal stroke (IPS) is common, resulting in significant mortality and morbidity. In such cases, the incidence of unilateral arterial cerebral infarction is often occluded in the middle cerebral artery (MCA), leading to focal ischemia. In adult rodents, blockage of MCA is the most frequently used strategy for ischemic stroke study. However, modeling MCA occlusion (MCAo) in postnatal day 0–7 (P0–7) mouse pups for IPS study has not been accomplished. Here we occluded the dMCA by inducing the accumulation of magnetic particles (MPs) administered through the superficial temporal vein of mice between P0 and P7, which we called neonatal or perinatal SIMPLE (Stroke Induced with Magnetic Particles). SIMPLE produced either permanent or transient occlusion in the dMCA of perinatal and neonatal mice. Permanent MCA occlusion with SIMPLE resulted in cerebral infarction and neuronal death in the brain. SIMPLE can also be used to reliably produce focal ischemic stroke in neonatal or perinatal mouse brains. As a result, SIMPLE allows the modeling of IPS or focal ischemic stroke for further mechanistic studies in mice, with particular utility for mimicking transient focal ischemia in human pre-term babies, which for the first time here has been accomplished in mice. The online version of this article (10.1186/s13041-018-0389-0) contains supplementary material, which is available to authorized users.
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DOI：
10.1523/jneurosci.2102-11.2011
发表时间：
2011-09-07
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Faustino JV;Wang X;Johnson CE;Klibanov A;Derugin N;Wendland MF;Vexler ZS
通讯作者：
Vexler ZS
影响因子：
3
作者：
Lynch, John Kylan
通讯作者：
Lynch, John Kylan
影响因子：
1.4
作者：
Mercuri, E;Jongmans, M;Dubowitz, L
通讯作者：
Dubowitz, L
影响因子：
48
作者：
Jia JM;Chowdary PD;Gao X;Ci B;Li W;Mulgaonkar A;Plautz EJ;Hassan G;Kumar A;Stowe AM;Yang SH;Zhou W;Sun X;Cui B;Ge WP
通讯作者：
Ge WP
影响因子：
8.3
作者：
Mallard C;Vexler ZS
通讯作者：
Vexler ZS