No evidence for Fabaceae Gametophytic self-incompatibility being determined by Rosaceae, Solanaceae, and Plantaginaceae S-RNase lineage genes.
No evidence for Fabaceae Gametophytic self-incompatibility being determined by Rosaceae, Solanaceae, and Plantaginaceae S-RNase lineage genes.
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DOI：
10.1186/s12870-015-0497-2
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发表时间：
2015-06-02
影响因子：
5.3
通讯作者：
Vieira CP
中科院分区：
文献类型：
--
作者：
Aguiar B;Vieira J;Cunha AE;Vieira CP
蚕豆科植物在农艺学和畜牧业中占有重要地位。它们有很长的育种历史，大多数品种已经失去了自交不亲和性(SI)，这是自交受精的遗传障碍。然而，为了改善豆科作物的育种，经常与栽培品种的野生SI近缘杂交。因此，对豆科植物的SI系统(S)进行研究是十分必要的。我们通过招募蔷薇科、茄科或车前科植物的相同的S-核糖核酸酶谱系基因，解决了蚕豆科配子体(G)SI是以核糖核酸酶为基础的假设。我们首先在红三叶、紫花苜蓿、西洋参、大豆和羽扇豆属的基因组中发现了SSK1类基因(仅在具有基于RNase的GSI的物种中描述)。然后，我们对这些基因组中的S系T2-核糖核酸酶基因进行了特征分析。在草地早熟禾、元宝菇和松材线虫中，我们鉴定了S-核糖核酸酶的谱系基因，在系统发育分析中，这些基因与松茸亚科的S-核糖核酸酶聚在一起。在可获得大支架的元宝枫和紫花锦鸡儿基因组中，这些序列被F-box基因包围，在系统发育分析中，F-box基因也与S花粉基因聚在一起。然而，在草地早熟禾中，S-核糖核酸酶谱系基因在与GSI无关的组织中表达。此外，S核糖核酸酶基因的多样性水平低于观察到的其他基因。与松茸亚科S-核糖核酸酶和S-花粉样基因亲缘关系较近的元宝菇和松茸花粉样基因，也在其他组织中表达，而不是在相关组织中表达。为了解决其他T2-RNase是否可能决定Fabaceae GSI的问题，我们在这里获得了一个带有柱头转录组的花柱，这是一个在自花授粉和异花授粉中花粉生长百分比存在显著差异的物种。对纹状体S-核糖核酸酶类似基因的表达和多态性分析表明，这些基因都不是S-雌蕊基因。我们没有发现由蔷薇科、茄科和车前科S核糖核酸酶谱系基因决定的豆科植物GSI的证据。没有证据表明T2-核糖核酸酶谱系基因可以决定条纹纹状体中的GSI。因此，为了研究蚕豆科S雌蕊基因(S)的特征，需要对那些在发生GSI的组织中高表达的基因进行控制杂交的表达分析、多样性水平和分离分析。本文的在线版本(doi：10.1186/s12870-0150497-2)包含补充材料，授权用户可以使用。
Fabaceae species are important in agronomy and livestock nourishment. They have a long breeding history, and most cultivars have lost self-incompatibility (SI), a genetic barrier to self-fertilization. Nevertheless, to improve legume crop breeding, crosses with wild SI relatives of the cultivated varieties are often performed. Therefore, it is fundamental to characterize Fabaceae SI system(s). We address the hypothesis of Fabaceae gametophytic (G)SI being RNase based, by recruiting the same S-RNase lineage gene of Rosaceae, Solanaceae or Plantaginaceae SI species. We first identify SSK1 like genes (described only in species having RNase based GSI), in the Trifolium pratense, Medicago truncatula, Cicer arietinum, Glycine max, and Lupinus angustifolius genomes. Then, we characterize the S-lineage T2-RNase genes in these genomes. In T. pratense, M. truncatula, and C. arietinum we identify S-RNase lineage genes that in phylogenetic analyses cluster with Pyrinae S-RNases. In M. truncatula and C. arietinum genomes, where large scaffolds are available, these sequences are surrounded by F-box genes that in phylogenetic analyses also cluster with S-pollen genes. In T. pratense the S-RNase lineage genes show, however, expression in tissues not involved in GSI. Moreover, levels of diversity are lower than those observed for other S-RNase genes. The M. truncatula and C. arietinum S-RNase and S-pollen like genes phylogenetically related to Pyrinae S-genes, are also expressed in tissues other than those involved in GSI. To address if other T2-RNases could be determining Fabaceae GSI, here we obtained a style with stigma transcriptome of Cytisus striatus, a species that shows significant difference on the percentage of pollen growth in self and cross-pollinations. Expression and polymorphism analyses of the C. striatus S-RNase like genes revealed that none of these genes, is the S-pistil gene. We find no evidence for Fabaceae GSI being determined by Rosaceae, Solanaceae, and Plantaginaceae S-RNase lineage genes. There is no evidence that T2-RNase lineage genes could be determining GSI in C. striatus. Therefore, to characterize the Fabaceae S-pistil gene(s), expression analyses, levels of diversity, and segregation analyses in controlled crosses are needed for those genes showing high expression levels in the tissues where GSI occurs. The online version of this article (doi:10.1186/s12870-015-0497-2) contains supplementary material, which is available to authorized users.
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