Intrinsic expression of host genes and intronic miRNAs in prostate carcinoma cells.
Intrinsic expression of host genes and intronic miRNAs in prostate carcinoma cells.
复制标题
DOI：
10.1186/1475-2867-9-21
复制
发表时间：
2009-08-12
影响因子：
5.8
通讯作者：
Shukla GC
中科院分区：
文献类型：
--
作者：
Sikand K;Slane SD;Shukla GC
最近的数据显示，在前列腺癌（PCa）中，调节性非编码微rna （mi）表达异常和改变。大量的mirna在许多蛋白质编码基因中以有组织的内含子簇的形式编码。虽然mirna的表达谱研究很普遍，但对宿主基因及其驻留的mirna在PCa细胞中的协调表达知之甚少。此外，在雄激素应答细胞和雄激素非依赖性细胞中，一组mirna的表达是否不同尚不清楚。本文研究了miR 17-92、miR 106b-25和miR 23b-24集群成熟mirna及其宿主基因C13orf25、MCM7和AMPO在PCa细胞系中的表达。在雄激素敏感型MDA PCa 2b和LNCaP以及雄激素难耐型PC-3和DU 145细胞培养模型中进行miRNAs和宿主基因的表达谱分析。miRNA的表达与PCa细胞内禀激素反应性无显著相关性。雄激素敏感的MDA PCa 2b细胞显示了本报告研究的大多数mirna的最高表达水平。我们发现宿主基因及其驻留mirna之间存在显著的表达差异。C13orf25与miR 17-92集群、MCM7与miR 106b-25集群的表达没有统计学上的显著相关性，提示宿主基因和驻留mirna可能是相互独立表达的。我们的研究结果表明，miRNA表达谱可能无法预测PCa细胞的内在激素敏感环境。更重要的是，我们的数据表明了PCa细胞中内含子miRNA加工的其他新机制的可能性。
Recent data show aberrant and altered expression of regulatory noncoding micro (mi) RNAs in prostate cancer (PCa). A large number of miRNAs are encoded in organized intronic clusters within many protein coding genes. While expression profiling studies of miRNAs are common place, little is known about the host gene and their resident miRNAs coordinated expression in PCa cells. Furthermore, whether expression of a subset of miRNAs is distinct in androgen-responsive and androgen-independent cells is not clear. Here we have examined the expression of mature miRNAs of miR 17–92, miR 106b-25 and miR 23b-24 clusters along with their host genes C13orf25, MCM7 and AMPO respectively in PCa cell lines. The expression profiling of miRNAs and host genes was performed in androgen-sensitive MDA PCa 2b and LNCaP as well as in androgen-refractory PC-3 and DU 145 cell culture models of PCa. No significant correlation between the miRNA expression and the intrinsic hormone-responsive property of PCa cells was observed. Androgen-sensitive MDA PCa 2b cells exhibited the highest level of expression of most miRNAs studied in this report. We found significant expression variations between host genes and their resident miRNAs. The expressions of C13orf25 and miR 17–92 cluster as well as MCM7 and miR 106b-25 cluster did not reveal statistically significant correlation, thus suggesting that host genes and resident miRNAs may be expressed independent of each other. Our results suggest that miRNA expression profiles may not predict intrinsic hormone-sensitive environment of PCa cells. More importantly, our data indicate the possibility of additional novel mechanisms for intronic miRNA processing in PCa cells.
登录
查看更多内容
影响因子：
64.8
作者：
Baek, Daehyun;Villen, Judit;Shin, Chanseok;Camargo, Fernando D.;Gygi, Steven P.;Bartel, David P.
通讯作者：
Bartel, David P.
影响因子：
5.3
作者：
Hossain, Anwar;Kuo, Macus T.;Saunders, Grady F.
通讯作者：
Saunders, Grady F.
Mirbase：MicroRNA基因组学的工具。
影响因子：
14.9
作者：
Griffiths-Jones, Sam;Saini, Harpreet Kaur;van Dongen, Stijn;Enright, Anton J.
通讯作者：
Enright, Anton J.
DOI：
10.1016/j.urolonc.2007.01.019
发表时间：
2007-09-01
影响因子：
2.7
作者：
Gottardo, Fedra;Liu, Chang Gong;Baffa, Raffaele
通讯作者：
Baffa, Raffaele
DOI：
10.1073/pnas.0803304105
发表时间：
2008-09-02
影响因子：
11.1
作者：
Foekens, John A.;Sieuwerts, Anieta M.;Martens, John W. M.
通讯作者：
Martens, John W. M.