Quantitative microbial faecal source tracking with sampling guided by hydrological catchment dynamics.
Quantitative microbial faecal source tracking with sampling guided by hydrological catchment dynamics.
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DOI：
10.1111/j.1462-2920.2008.01682.x
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发表时间：
2008-10
影响因子：
5.1
通讯作者：
Farnleitner AH
中科院分区：
文献类型：
--
作者：
Reischer GH;Haider JM;Sommer R;Stadler H;Keiblinger KM;Hornek R;Zerobin W;Mach RL;Farnleitner AH
粪便污染对水质的损害是一个全球性的公共卫生问题。微生物源跟踪方法有助于确定粪便来源，但最近的几个定量微生物源跟踪应用忽视了流域水文和污染动力学。这种定量的微生物源跟踪研究，在一个大型岩溶泉流域进行，可能受到人类和反刍动物的影响，是基于分层采样方法：31个月的水质监测（监测），包括季节性水文动态和洪水事件（事件）的调查，作为最强的污染时期。通过定量实时PCR检测反刍动物特异性和人类特异性粪便拟杆菌标志物，并辅以标准微生物和在线水文参数。在监测和事件期间，在泉水中检测到两种定量微生物源跟踪标记，其中反刍动物特异性标记占优势。对所有数据进行多参数分析，可以将反刍动物特异性标记与一般粪便污染指标联系起来，特别是在事件期间。高达80%的事件期间的粪便指标水平的变化可以解释反刍动物特异性标记水平证明反刍动物粪便来源的优势，在集水区。此外，排除了土壤作为定量微生物源跟踪标记物的来源。本研究论证了定量微生物源追踪方法的适用性，并强调了在源追踪研究设计中考虑水文流域动态的前提。
The impairment of water quality by faecal pollution is a global public health concern. Microbial source tracking methods help to identify faecal sources but the few recent quantitative microbial source tracking applications disregarded catchment hydrology and pollution dynamics. This quantitative microbial source tracking study, conducted in a large karstic spring catchment potentially influenced by humans and ruminant animals, was based on a tiered sampling approach: a 31-month water quality monitoring (Monitoring) covering seasonal hydrological dynamics and an investigation of flood events (Events) as periods of the strongest pollution. The detection of a ruminant-specific and a human-specific faecal Bacteroidetes marker by quantitative real-time PCR was complemented by standard microbiological and on-line hydrological parameters. Both quantitative microbial source tracking markers were detected in spring water during Monitoring and Events, with preponderance of the ruminant-specific marker. Applying multiparametric analysis of all data allowed linking the ruminant-specific marker to general faecal pollution indicators, especially during Events. Up to 80% of the variation of faecal indicator levels during Events could be explained by ruminant-specific marker levels proving the dominance of ruminant faecal sources in the catchment. Furthermore, soil was ruled out as a source of quantitative microbial source tracking markers. This study demonstrates the applicability of quantitative microbial source tracking methods and highlights the prerequisite of considering hydrological catchment dynamics in source tracking study design.
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影响因子：
4.4
作者：
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通讯作者：
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