Accuracy of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Mycobacteria: a systematic review and meta-analysis.
Accuracy of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Mycobacteria: a systematic review and meta-analysis.
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基质辅助激光解吸电离飞行时间质谱法鉴定分枝杆菌的准确性：系统评价和荟萃分析。
DOI：
10.1038/s41598-018-22642-w
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发表时间：
2018-03-07
影响因子：
4.6
通讯作者：
Ju S
中科院分区：
文献类型：
--
作者：
Cao Y;Wang L;Ma P;Fan W;Gu B;Ju S
分枝杆菌是世界范围内发病率和死亡率的重要原因。本研究旨在系统评价基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴别临床致病性分枝杆菌的准确性。经过严格的筛选过程，纳入了19篇涉及2593株分枝杆菌分离株的文章。合并结果与参考方法鉴定结果一致，85%的菌株在属水平上与参考方法鉴定结果一致，71% （95% CI为69% ~ 72%）的菌株在种水平上与参考方法鉴定结果一致。MALDI-TOF MS在物种水平上正确识别了92%的结核分枝杆菌（95% CI为0.87 ~ 0.96）和68%的牛分枝杆菌（95% CI为27% ~ 100%）。固体培养基中结核分枝杆菌复合体与参考菌株使用增强数据库显示更准确的识别。biomrieuxvitek MS的鉴定准确率略高于Bruker MALDI Biotyper （75% vs 72%）。然而，这两种系统对福氏分枝杆菌、kansasii分枝杆菌、marinum分枝杆菌和terrae分枝杆菌的鉴定结果却相反。综上所述，我们的结果表明MALDI-TOF质谱在临床致病性分枝杆菌鉴定中的应用尚不令人满意。日益增长的改进需求是重要的，特别是在物种水平上。
Mycobacterium species are a significant cause of morbidity and mortality worldwide. The present study was carried out to systematically evaluate the accuracy of Matrix-assisted laser desorption ionization–time of flight mass spectroscopy (MALDI-TOF MS) for the identification of clinical pathogenic mycobacteria. After a rigid selection process, 19 articles involving 2,593 mycobacteria isolates were included. The pooled result agreed with the reference method identification for 85% of the isolates to genus level, with 71% (95% CI of 69% to 72%) correct to the species level. The MALDI-TOF MS correctly identified 92% of the M.tuberculosis isolates (95% CI of 0.87 to 0.96), and 68% of M. bovisisolates (95% CI of 27% to 100%) to the species level. Mycobacterium tuberculosis complex in solid media with reference strains using augmented database showing more accurate identification. The identifying accuracy rate of bioMérieuxVitek MS was slight higher than Bruker MALDI Biotyper (75% vs 72%). However, opposite results were obtained in identifications of M. fortuitum, M. kansasii, M. marinum, and M. terrae with these two systems. In summary, our results demonstrate that application of MALDI-TOF MS in clinical pathogenic mycobacteria identification is less satisfactory to date. Increasing need for improvement is important especially at species level.
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影响因子：
4.1
作者：
Kohlmann, Rebekka;Hoffmann, Alexander;Gatermann, Soeren
通讯作者：
Gatermann, Soeren
DOI：
10.5588/ijtld.12.0843
发表时间：
2013-06-01
影响因子：
4
作者：
Jankovic, M.;Samarzija, M.;van Ingen, J.
通讯作者：
van Ingen, J.
影响因子：
1.5
作者：
Hines, N;Payeur, JB;Hoffman, LJ
通讯作者：
Hoffman, LJ
影响因子：
3.7
作者：
El Khéchine A;Couderc C;Flaudrops C;Raoult D;Drancourt M
通讯作者：
Drancourt M
影响因子：
9.4
作者：
Fangous, Marie-Sarah;Mougari, Faiza;Hery-Arnaud, Genevieve
通讯作者：
Hery-Arnaud, Genevieve