De novo genome and transcriptome analyses provide insights into the biology of the trematode human parasite Fasciolopsis buski.
De novo genome and transcriptome analyses provide insights into the biology of the trematode human parasite Fasciolopsis buski.
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DOI：
10.1371/journal.pone.0205570
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Tandon V
中科院分区：
文献类型：
--
作者：
Biswal DK;Roychowdhury T;Pandey P;Tandon V
许多吸虫寄生虫引起人类感染，被认为是一个主要的公共卫生问题。它们在不同地区的生态多样性为这些生物的进化提供了挑战性的问题。在这份报告中，我们使用下一代测序技术对巨肠吸虫姜片虫进行转录组分析。将来源于该生物体的含有polyA的RNA的短读段序列组装成30，677个单基因，导致12，380个基因的注释。对这些转录本的注释使我们能够深入了解肠吸虫的过程和途径，如RNAi途径和能量代谢。通过鉴定所有蛋白激酶来表征生物体的表达的激酶组。本文还报道了姜片虫基因组组装的粗略草图，其中SRA加入用于交叉检查分析的转录组数据中的发现。转录组数据也帮助我们鉴定了一些表达的转座因子。虽然识别出许多长散布元素（LINE），但只有两个短散布元素（西内斯）可见。对F. Buski帮助我们描述了它的一些重要生物学特性，并为开发合适的诊断系统和抗寄生虫治疗分子提供了巨大的资源。
Many trematode parasites cause infection in humans and are thought to be a major public health problem. Their ecological diversity in different regions provides challenging questions on evolution of these organisms. In this report, we perform transcriptome analysis of the giant intestinal fluke, Fasciolopsis buski, using next generation sequencing technology. Short read sequences derived from polyA containing RNA of this organism were assembled into 30,677 unigenes that led to the annotation of 12,380 genes. Annotation of the assembled transcripts enabled insight into processes and pathways in the intestinal fluke, such as RNAi pathway and energy metabolism. The expressed kinome of the organism was characterized by identifying all protein kinases. A rough draft genome assembly for Fasciolopsis buski is also reported herewith with SRA accessions for crosschecking the findings in the analyzed transcriptome data. Transcriptome data also helped us to identify some of the expressed transposable elements. Though many Long Interspersed elements (LINEs) were identified, only two Short Interspersed Elements (SINEs) were visible. Overall transcriptome and draft genome analysis of F. buski helped us to characterize some of its important biological characteristics and provided enormous resources for development of a suitable diagnostic system and anti-parasitic therapeutic molecules.
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影响因子：
4.4
作者：
Andrade LF;Nahum LA;Avelar LG;Silva LL;Zerlotini A;Ruiz JC;Oliveira G
通讯作者：
Oliveira G
巨型肠fluke的完整线粒体基因组的下一代测序和注释的集成管道，fascioloppis buski（Lankester，1857年），1899年。
影响因子：
2.7
作者：
Biswal DK;Ghatani S;Shylla JA;Sahu R;Mullapudi N;Bhattacharya A;Tandon V
通讯作者：
Tandon V
影响因子：
14.9
作者：
UniProt Consortium
通讯作者：
UniProt Consortium
影响因子：
12.3
作者：
Conesa A;Madrigal P;Tarazona S;Gomez-Cabrero D;Cervera A;McPherson A;Szcześniak MW;Gaffney DJ;Elo LL;Zhang X;Mortazavi A
通讯作者：
Mortazavi A
影响因子：
5.8
作者：
Conesa, A;Götz, S;Robles, M
通讯作者：
Robles, M