Population genomics supports baculoviruses as vectors of horizontal transfer of insect transposons.
Population genomics supports baculoviruses as vectors of horizontal transfer of insect transposons.
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DOI：
10.1038/ncomms4348
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发表时间：
2014
影响因子：
16.6
通讯作者：
Cordaux, Richard
中科院分区：
文献类型：
--
作者：
Gilbert, Clement;Chateigner, Aurelien;Ernenwein, Lise;Barbe, Valerie;Bezier, Annie;Herniou, Elisabeth A.;Cordaux, Richard
DNA水平转移（HT）是影响真核生物进化的重要因素。虽然已经报道了数百个真核间转座因子（te）的ht，但这些转移的载体仍然难以捉摸。本研究表明，在毛虫感染期间，来自卷心菜环虫（Trichoplusia ni）的两个te的多个拷贝在体内转位到加州自签名杆状病毒多核多角体病毒（AcMNPV）的基因组中。结果表明，同域飞蛾（T. ni, Manduca sexta, Helicoverpa spp）对AcMNPV具有不同程度的易感性。基于两个独立的种群基因组学数据集（总覆盖率达到330,000倍），我们报告了约8,500个AcMNPV基因组中一个月TE的频率。总之，我们的研究结果有力地支持了病毒作为动物间热传播媒介的作用，并呼吁系统地评估病毒介导的热传播频率及其对宿主基因组进化的影响。DNA的水平转移在真核生物中很常见，但涉及的载体仍然难以捉摸。在这里，Gilbert等人展示了高频率的体内转位，从白菜环蛾到杆状病毒的基因组，这表明病毒可以作为动物之间水平转移的载体。
Horizontal transfer (HT) of DNA is an important factor shaping eukaryote evolution. Although several hundreds of eukaryote-to-eukaryote HTs of transposable elements (TEs) have been reported, the vectors underlying these transfers remain elusive. Here, we show that multiple copies of two TEs from the cabbage looper (Trichoplusia ni) transposed in vivo into genomes of the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) during caterpillar infection. We further demonstrate that both TEs underwent recent HT between several sympatric moth species (T. ni, Manduca sexta, Helicoverpa spp.) showing different degrees of susceptibility to AcMNPV. Based on two independent population genomics data sets (reaching a total coverage >330,000X), we report a frequency of one moth TE in ~8,500 AcMNPV genomes. Together, our results provide strong support for the role of viruses as vectors of TE HT between animals, and they call for a systematic evaluation of the frequency and impact of virus-mediated HT on the evolution of host genomes. Horizontal transfer of DNA is common among eukaryotes but the vectors involved remain elusive. Here, Gilbert et al. show high frequency of in vivo transposition from the cabbage looper moth into genomes of a baculovirus, suggesting that viruses can act as vectors of horizontal transfer between animals.
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