Rice XB15, a protein phosphatase 2C, negatively regulates cell death and XA21-mediated innate immunity.
Rice XB15, a protein phosphatase 2C, negatively regulates cell death and XA21-mediated innate immunity.
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DOI：
10.1371/journal.pbio.0060231
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发表时间：
2008-09-23
期刊：
影响因子：
9.8
通讯作者：
Ronald PC
中科院分区：
文献类型：
--
作者：
Park CJ;Peng Y;Chen X;Dardick C;Ruan D;Bart R;Canlas PE;Ronald PC
细胞表面受体对细胞外信号的感知对于真核生物的发育和免疫具有重要意义。与受体相关的激酶或作为受体本身的一部分的激酶通过磷酸化事件调节信号传导。水稻（Oryza sativa L.）XA 21受体激酶是先天免疫应答中的关键识别和信号传导决定因素。酵母双杂交筛选使用XA 21的细胞内部分，包括质膜（JM）和激酶结构域作为诱饵，鉴定了蛋白磷酸酶2C（PP 2C），称为XA 21结合蛋白15（XB 15）。分别通过谷胱甘肽-S-转移酶（GST）下拉和免疫共沉淀试验在体外和体内证实了XA 21和XB 15的相互作用。从大肠杆菌和转基因水稻中纯化的XB 15融合蛋白具有PP 2C活性。自身磷酸化的XA 21可以通过XB 15以时间和剂量依赖性方式去磷酸化。XA 21 JM结构域中的丝氨酸残基是XB 15结合所必需的。Xb 15突变体表现出严重的细胞死亡表型，诱导病程相关基因，并增强了XA 21介导的抗性。Xb 15在XA 21水稻品系中的过表达损害了对细菌病原体黄单胞菌的抗性。米。这些结果表明，Xb 15编码PP 2C，其负调节XA 21介导的先天免疫应答。对病原体的抵抗力对植物和动物的生存至关重要。与动物不同，植物缺乏适应性免疫系统，而是依靠先天免疫反应来抵御感染。为了阐明植物先天免疫的分子机制，我们正在研究由水稻病原体识别受体激酶XA 21介导的信号级联，该激酶赋予对细菌病原体黄单胞菌的抗性。米。我们证明，XA 21结合蛋白15（蛋白磷酸酶2C）负调控XA 21介导的信号传导阻力。这一发现为受体激酶介导的免疫调节提供了重要的见解。水稻蛋白磷酸酶2C XB 15是病原体识别受体激酶介导的细胞死亡和先天免疫的负调节因子。
Perception of extracellular signals by cell surface receptors is of central importance to eukaryotic development and immunity. Kinases that are associated with the receptors or are part of the receptors themselves modulate signaling through phosphorylation events. The rice (Oryza sativa L.) XA21 receptor kinase is a key recognition and signaling determinant in the innate immune response. A yeast two-hybrid screen using the intracellular portion of XA21, including the juxtamembrane (JM) and kinase domain as bait, identified a protein phosphatase 2C (PP2C), called XA21 binding protein 15 (XB15). The interaction of XA21 and XB15 was confirmed in vitro and in vivo by glutathione-S-transferase (GST) pull-down and co-immunoprecipitation assays, respectively. XB15 fusion proteins purified from Escherichia coli and from transgenic rice carry PP2C activity. Autophosphorylated XA21 can be dephosphorylated by XB15 in a temporal- and dosage-dependent manner. A serine residue in the XA21 JM domain is required for XB15 binding. Xb15 mutants display a severe cell death phenotype, induction of pathogenesis-related genes, and enhanced XA21-mediated resistance. Overexpression of Xb15 in an XA21 rice line compromises resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae. These results demonstrate that Xb15 encodes a PP2C that negatively regulates the XA21-mediated innate immune response. Resistance to pathogens is critical to plant and animal survival. Plants, unlike animals, lack an adaptive immune system and instead rely on the innate immune response to protect against infection. To elucidate the molecular mechanism of plant innate immunity, we are studying the signaling cascade mediated by the rice pathogen recognition receptor kinase XA21, which confers resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae. We demonstrate that XA21 binding protein 15 (a protein phosphatase 2C) negatively regulates XA21-mediated signaling resistance. This finding provides significant insight into regulation of receptor kinase-mediated immunity. The rice protein phosphatase 2C XB15 is a negative regulator of pathogen recognition receptor kinase-mediated cell death and innate immunity.
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影响因子：
5.1
作者：
Bart R;Chern M;Park CJ;Bartley L;Ronald PC
通讯作者：
Ronald PC
影响因子：
2.7
作者：
HOISINGTON, DA;NEUFFER, MG;WALBOT, V
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WALBOT, V
影响因子：
11.6
作者：
Gómez-Gómez, L;Bauer, Z;Boller, T
通讯作者：
Boller, T
影响因子：
30.5
作者：
Ausubel, FM
通讯作者：
Ausubel, FM
影响因子：
64.8
作者：
Asai, T;Tena, G;Sheen, J
通讯作者：
Sheen, J