Loosening and reorganization of fluid phospholipid bilayers by chloroform.
Loosening and reorganization of fluid phospholipid bilayers by chloroform.
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DOI：
10.1021/ja9011468
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发表时间：
2009-04-15
影响因子：
15
通讯作者：
Regen SL
中科院分区：
文献类型：
--
作者：
Turkyilmaz S;Chen WH;Mitomo H;Regen SL
通过最近邻识别（NNR）方法，研究了由含有不同浓度胆固醇的1,2-二棕榈酰-sn-甘油-3-磷脂胆碱（DPPC）制成的宿主双层中可交换磷脂(A)与可交换甾醇(B)的混合行为。在低固醇浓度（即2.5 mol%）下，A和B的混合接近理想。当甾醇浓度增加到40 mol%时，导致A和b之间的亲和力净增加。在氯仿存在的情况下进行的类似混合实验显示出平衡效应，在所有情况下都观察到适度的甾醇-磷脂亲和力。这些结果，加上每个磷脂吸收的氯仿分子的数量基本上是恒定的，与甾醇含量无关，支持了一个模型，在这个模型中氯仿有利于磷脂的溶剂化，并产生了共同的膜状态。荧光测量和拉曼光谱也显示氯仿显著地使DPPC制成的低胆固醇和富胆固醇的膜松动。在更广泛的背景下，这些结果表明了一种全新的麻醉机制，其中麻醉剂通过溶剂化脂质成分，深刻地改变了脂质框架的横向组织。
The mixing behavior of an exchangeable phospholipid (A) with an exchangeable sterol (B) has been examined in host bilayers made from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), containing varying concentrations of cholesterol, via the nearest-neighbor recognition (NNR) method. At low sterol concentrations (i.e., 2.5 mol%), the mixing between A and B is close to ideal. Incremental increases in sterol concentrations to 40 mol% led to net increases in the affinity between A and B. Similar mixing experiments that were carried out in the presence of chloroform showed a leveling effect, where moderate sterol-phospholipid affinity was observed in all cases. These results, together with the fact that the number of chloroform molecules that are absorbed per phospholipid is essentially constant and independent of the sterol content, supports a model in which chloroform favors solvation of the phospholipids and a common membrane state is produced. Fluorescence measurements and Raman spectra have also shown that chloroform significantly loosens both cholesterol-poor and cholesterol-rich membranes made from DPPC. In a broader context, these results suggest a fundamentally new mechanim of anesthesia, where the anesthetic, by solvating the lipid components, profoundly changes the lateral organization of the lipid framework.
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DOI：
10.1016/0005-2736(77)90078-5
发表时间：
1977-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
GABER, BP;PETICOLAS, WL
通讯作者：
PETICOLAS, WL
影响因子：
15
作者：
Sugahara, M;Uragami, M;Regen, SL
通讯作者：
Regen, SL
影响因子：
3.4
作者：
Bondarenko, Vasyl;Yushmanov, Victor E.;Tang, Pei
通讯作者：
Tang, Pei
影响因子：
15
作者：
Cao, HH;Zhang, JB;Regen, SL
通讯作者：
Regen, SL
影响因子：
3.3
作者：
Chiang, Yun-Wei;Costa, Antonio J.;Freed, Jack H.
通讯作者：
Freed, Jack H.