Phenotypic, Transcriptomic, and Metabolomic Signatures of Root-Specifically Overexpressed OsCKX2 in Rice.
Phenotypic, Transcriptomic, and Metabolomic Signatures of Root-Specifically Overexpressed OsCKX2 in Rice.
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水稻根部特异性过表达 OsCKX2 的表型、转录组学和代谢组学特征
DOI：
10.3389/fpls.2020.575304
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发表时间：
2020
影响因子：
5.6
通讯作者：
Zhao Q
中科院分区：
文献类型：
--
作者：
Yan H;Sun H;Jia X;Lv C;Li J;Zhao Q
细胞分裂素是调节植物生长发育的重要信号分子。OsCKX 2通过编码细胞分裂素氧化酶/脱氢酶不可逆地降解核碱基细胞分裂素来控制水稻的籽粒产量。为了研究根源细胞分裂素对水稻生长的影响，本研究利用RCc 3启动子在水稻根中特异性表达OsCKX 2。OsCKX 2过表达（OE）水稻表现出生长迟缓，细胞分裂素水平和生物量产量较低。OsCKX 2 OE水稻和野生型（WT）之间的茎特异性转录组分析揭示了与细胞分裂、细胞壁结构、植物激素信号传导以及同化和催化相关的差异表达基因（DEG）。代谢物组学分析表明，OsCKX 2 OE水稻的氨基酸和有机酸等主要初级代谢产物含量增加，而脂类含量减少。整合转录组学和代谢组学数据显示，几个DEG和差异代谢产物与糖酵解和三羧酸循环（TCA）有关。综上所述，通过OsCKX 2的根特异性过表达降低细胞分裂素水平导致发育缺陷，这证实了根源细胞分裂素在植物生长和形态建成中的重要性。
Cytokinins are crucial signaling molecules that regulate plant growth and development. OsCKX2 irreversibly degrades nucleobase cytokinins by encoding cytokinin oxidase/dehydrogenase to control grain production in rice. In this study, OsCKX2 was specifically overexpressed in roots using RCc3 promoter to investigate the effects of root-source cytokinins on the growth of rice. OsCKX2 overexpressed (OE) rice showed retarded growth with lower cytokinin levels and biomass production. Shoot-specific transcriptome analysis between OsCKX2 OE rice and wild type (WT) revealed differentially expressed genes (DEGs) associated with cell division, cell wall structure, phytohormone signaling, and assimilation and catabolism. Metabolome analysis indicated that a majority of differential primary metabolites, such as amino acids and organic acids, increased, while lipids decreased in OsCKX2 OE rice. Integration of transcriptomic and metabolomic data showed that several DEGs and differential metabolites were related to glycolysis and tricarboxylic acid cycle (TCA). To conclude, reduced cytokinin levels via root-specific overexpression of OsCKX2 resulted in developmental defects, which confirmed the importance of root-source cytokinins in plant growth and morphogenesis.
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DOI：
10.2147/rrb.s84753
发表时间：
2015-01-01
期刊：
RESEARCH AND REPORTS IN BIOLOGY
影响因子：
--
作者：
Boycheva, Irina;Vassileva, Valya;Iantcheva, Anelia
通讯作者：
Iantcheva, Anelia
影响因子：
6.9
作者：
Julius BT;Slewinski TL;Baker RF;Tzin V;Zhou S;Bihmidine S;Jander G;Braun DM
通讯作者：
Braun DM
影响因子：
4.6
作者：
Gigli-Bisceglia, Nora;Engelsdorf, Timo;Hamann, Thorsten
通讯作者：
Hamann, Thorsten
影响因子：
5.1
作者：
Guo, Jing;Song, Jian;Zhang, Xian Sheng
通讯作者：
Zhang, Xian Sheng
影响因子：
3.7
作者：
Nam YJ;Tran LS;Kojima M;Sakakibara H;Nishiyama R;Shin R
通讯作者：
Shin R