Transcriptional Analysis of the 5′-Noncoding Region of the Human Involucrin Gene (*)
Transcriptional Analysis of the 5′-Noncoding Region of the Human Involucrin Gene (*)
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人类外皮蛋白基因 5-非编码区的转录分析 (*)
DOI：
10.1074/jbc.271.1.512
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
L. M. Alvarez
中科院分区：
文献类型：
--
作者：
E. López;Alfonso Vega;A. Cadena;Sonia E. Granados;L. F. Jave;P. Gariglio;L. M. Alvarez
Human involucrin whose gene transcription is directed by a 2456-nucleotide (nt) 5′-noncoding region is a structural component of the epithelial cornified layer. Transient transfection assays demonstrated that this region is transcriptionally active in multiplying keratinocytes and is enhanced by 2 mM CaCl treatment. Calcium-independent transcriptional activity and the interaction with the AP-1 transcriptional factor was located on the proximal part (nt −159 to −1) of the 5′-noncoding region. However, CaCl responsiveness was mapped to a distal 1185-nt fragment (nt −2456 to −1272). Moreover, this fragment potentiated the Herpes simplex thymidine kinase promoter in normal keratinocytes and is responsive to calcium treatment in a cell type-specific manner. Interestingly, the absence of a 491-nt fragment located between the two enhancer domains (nt −651 to −160) resulted in transcriptional activation in multiplying keratinocytes. This fragment interacts with AP-1 and the YY1 transcriptional silencer. It is concluded that human involucrin 5′-noncoding region contains at least three regulatory domains, a distal CaCl-responsive enhancer, a putative transcriptional silencer (that interacts with AP-1 and YY1), and a proximal enhancer/promoter (that interacts with AP-1). Thus, this study demonstrates the presence of particular transcriptional factors can potentially regulate the human involucrin expression.
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使用瞬时 β-半乳糖苷酶测定法表征人外皮蛋白启动子。
影响因子：
4
作者：
Carroll,JM;Taichman,LB
通讯作者：
Taichman,LB
DOI：
10.1073/pnas.89.20.9814
发表时间：
1992-10-15
影响因子：
11.1
作者：
LEE, TC;SHI, Y;SCHWARTZ, RJ
通讯作者：
SCHWARTZ, RJ
影响因子：
56.9
作者：
RAUSCHER, FJ;COHEN, DR;FRANZA, BR
通讯作者：
FRANZA, BR
影响因子：
6.5
作者：
HOHL, D;LICHTI, U;ROOP, DR
通讯作者：
ROOP, DR
影响因子：
56.9
作者：
Ajay Shrivastava;S. Saleque;G. Kalpana;Steven E. Artandi;Stephen Goff;Kathryn Calame
通讯作者：
Ajay Shrivastava;S. Saleque;G. Kalpana;Steven E. Artandi;Stephen Goff;Kathryn Calame