Suppressing chlorophyll degradation by silencing OsNYC3 improves rice resistance to Rhizoctonia solani, the causal agent of sheath blight.
Suppressing chlorophyll degradation by silencing OsNYC3 improves rice resistance to Rhizoctonia solani, the causal agent of sheath blight.
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DOI：
10.1111/pbi.13715
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发表时间：
2022-03
影响因子：
13.8
通讯作者：
Zuo S
中科院分区：
文献类型：
--
作者：
Cao W;Zhang H;Zhou Y;Zhao J;Lu S;Wang X;Chen X;Yuan L;Guan H;Wang G;Shen W;De Vleesschauwer D;Li Z;Shi X;Gu J;Guo M;Feng Z;Chen Z;Zhang Y;Pan X;Liu W;Liang G;Yan C;Hu K;Liu Q;Zuo S
坏死性真菌solani Rhizoctonia k<e:1> (R. solani)在世界上许多作物中引起严重疾病，包括水稻和玉米纹枯病（ShB）。作物对真菌的抗性是一种数量性状，抗性机制在很大程度上仍然未知，严重阻碍了抗性品种的开发。在本研究中，我们发现在田间和室内条件下，抗性品种YSBR1对茄蚜的抑制能力明显强于敏感品种Lemont。转录组学比较表明，solani对Lemont的光合系统（包括叶绿素生物合成）有较高的抑制作用，而对YSBR1的抑制作用较弱。YSBR1的叶绿素含量高于Lemont，暗处理诱导叶绿素降解显著降低了其抗性。此外，3个携带受损叶绿素生物合成的水稻突变体和1个携带受损叶绿素生物合成的玉米突变体对茄蚜的敏感性均增强。叶绿素降解基因OsNYC3的过表达明显诱导了番茄红霉感染的表达，显著提高了高产ShB敏感品种9522对ShB的敏感性。然而，在田间试验中，沉默其转录明显提高了ShB抗性，而不影响农艺性状或产量。有趣的是，改变叶绿素含量并不影响水稻对白叶枯病和稻瘟病的抗性，这两种病分别由生物营养和半生物营养病原体引起。研究表明，叶绿素在水稻抗ShB抗性中起着重要的作用，抑制番茄枯萎病诱导的叶绿素降解可明显提高水稻对ShB的抗性。这一发现为开发抗坏死性真菌的作物提供了新的靶点。
Necrotrophic fungus Rhizoctonia solani Kühn (R. solani) causes serious diseases in many crops worldwide, including rice and maize sheath blight (ShB). Crop resistance to the fungus is a quantitative trait and resistance mechanism remains largely unknown, severely hindering the progress on developing resistant varieties. In this study, we found that resistant variety YSBR1 has apparently stronger ability to suppress the expansion of R. solani than susceptible Lemont in both field and growth chamber conditions. Comparison of transcriptomic profiles shows that the photosynthetic system including chlorophyll biosynthesis is highly suppressed by R. solani in Lemont but weakly in YSBR1. YSBR1 shows higher chlorophyll content than that of Lemont, and inducing chlorophyll degradation by dark treatment significantly reduces its resistance. Furthermore, three rice mutants and one maize mutant that carry impaired chlorophyll biosynthesis all display enhanced susceptibility to R. solani. Overexpression of OsNYC3, a chlorophyll degradation gene apparently induced expression by R. solani infection, significantly enhanced ShB susceptibility in a high‐yield ShB‐susceptible variety ‘9522’. However, silencing its transcription apparently improves ShB resistance without compromising agronomic traits or yield in field tests. Interestingly, altering chlorophyll content does not affect rice resistance to blight and blast diseases, caused by biotrophic and hemi‐biotrophic pathogens, respectively. Our study reveals that chlorophyll plays an important role in ShB resistance and suppressing chlorophyll degradation induced by R. solani infection apparently improves rice ShB resistance. This discovery provides a novel target for developing resistant crop to necrotrophic fungus R. solani.
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