Cultivation-independent methods reveal differences among bacterial gut microbiota in triatomine vectors of Chagas disease.
Cultivation-independent methods reveal differences among bacterial gut microbiota in triatomine vectors of Chagas disease.
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DOI：
10.1371/journal.pntd.0001631
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发表时间：
2012
影响因子：
3.8
通讯作者：
Azambuja P
中科院分区：
文献类型：
--
作者：
da Mota FF;Marinho LP;Moreira CJ;Lima MM;Mello CB;Garcia ES;Carels N;Azambuja P
查加斯病是一种锥虫病，其病原体是原生动物寄生虫克氏锥虫，它通过被称为锥虫的吸血虫传播给人类。尽管在南美锥虫病流行的大多数拉丁美洲国家，杀虫剂治疗可以有效控制这些虫子，但这种疾病仍然影响着南美洲很大一部分人口。该疾病在人类中的特征已被广泛研究，寄生虫的基因组已被测序，但尚未有有效的药物可用于治疗恰加斯病。昆虫的消化道，其中T。cruzi的研究远不如来自人类宿主的血液，它构成了一个条件非常不同的动态环境。因此，我们研究了来自Rhodnius属、Triatoma属、Panstrongylus属和Dipetalogaster属的昆虫载体中微生物群的优势细菌物种的组成。锥蝽肠道的微生物群使用不依赖培养的方法进行研究，即，利用变性梯度凝胶电泳（DGGE）和克隆测序进行16s rDNA的系统发育分析。超指数表明，锥蝽肠道细菌物种的多样性很低，包括不到20个优势种，这些物种之间的昆虫物种。结果表明，红腹小蜂属中以沙雷氏菌属为主，锥口小蜂属和泛圆线虫属中以砷音菌属为主，裂腹小蜂属中以罗氏小蜂属为主。锥蝽肠道菌群是干扰锥蝽的因素之一。克氏病毒在人类中的传播和毒力。了解其昆虫种类组成对设计生物防治措施具有重要意义。克鲁兹我们发现，锥蝽属中的细菌微生物群的优势种形成了一组低复杂性，其结构根据载体属而不同。恰加斯病是南美洲和中美洲最重要的地方病之一。它的病原体是原生动物克氏锥虫，它通过被称为锥蝽的吸血昆虫传播给人类。这些介体主要属于猎蝽科的Rhodnius属、Triatoma属和Panstrongylus属。这些载体肠道中的细菌群落可能对T.克鲁兹出于这个原因，我们分析了细菌的多样性托管在肠道中的不同种类的锥蝽使用培养独立的方法。在Rhodnius sp.中，我们从昆虫或森林条件下获得的样品中观察到类似的细菌群落。Arsenophonus属的内共生菌优先与泛圆线虫属和锥虫属的昆虫相关，而细菌属Serratia和Candidatus Rohrkolberia分别是Rhodnius和Dipetalogaster的典型。微生物群的多样性往往是最大的锥虫属，与Arsenophonus和Serratia的物种被检测到在T。害虫
Chagas disease is a trypanosomiasis whose agent is the protozoan parasite Trypanosoma cruzi, which is transmitted to humans by hematophagous bugs known as triatomines. Even though insecticide treatments allow effective control of these bugs in most Latin American countries where Chagas disease is endemic, the disease still affects a large proportion of the population of South America. The features of the disease in humans have been extensively studied, and the genome of the parasite has been sequenced, but no effective drug is yet available to treat Chagas disease. The digestive tract of the insect vectors in which T. cruzi develops has been much less well investigated than blood from its human hosts and constitutes a dynamic environment with very different conditions. Thus, we investigated the composition of the predominant bacterial species of the microbiota in insect vectors from Rhodnius, Triatoma, Panstrongylus and Dipetalogaster genera. Microbiota of triatomine guts were investigated using cultivation-independent methods, i.e., phylogenetic analysis of 16s rDNA using denaturing gradient gel electrophoresis (DGGE) and cloned-based sequencing. The Chao index showed that the diversity of bacterial species in triatomine guts is low, comprising fewer than 20 predominant species, and that these species vary between insect species. The analyses showed that Serratia predominates in Rhodnius, Arsenophonus predominates in Triatoma and Panstrongylus, while Candidatus Rohrkolberia predominates in Dipetalogaster. The microbiota of triatomine guts represents one of the factors that may interfere with T. cruzi transmission and virulence in humans. The knowledge of its composition according to insect species is important for designing measures of biological control for T. cruzi. We found that the predominant species of the bacterial microbiota in triatomines form a group of low complexity whose structure differs according to the vector genus. Chagas disease is one of the most important endemic diseases of South and Central America. Its causative agent is the protozoan Trypanosoma cruzi, which is transmitted to humans by blood-feeding insects known as triatomine bugs. These vectors mainly belong to Rhodnius, Triatoma and Panstrongylus genera of Reduviidae. The bacterial communities in the guts of these vectors may have important effects on the biology of T. cruzi. For this reason, we analyzed the bacterial diversity hosted in the gut of different species of triatomines using cultivation-independent methods. Among Rhodnius sp., we observed similar bacterial communities from specimens obtained from insectaries or sylvatic conditions. Endosymbionts of the Arsenophonus genus were preferentially associated with insects of the Panstrongylus and Triatoma genera, whereas the bacterial genus Serratia and Candidatus Rohrkolberia were typical of Rhodnius and Dipetalogaster, respectively. The diversity of the microbiota tended to be the largest in the Triatoma genus, with species of both Arsenophonus and Serratia being detected in T. infestans.
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DOI：
10.4269/ajtmh.2008.79.881
发表时间：
2008-12-01
影响因子：
3.3
作者：
Hillesland, Heidi;Read, Amber;Durvasula, Ravi
通讯作者：
Durvasula, Ravi
影响因子：
4
作者：
Beard, CB;Dotson, EM;Durvasula, RV
通讯作者：
Durvasula, RV
DOI：
10.1590/s0074-02762007005000098
发表时间：
2007-10-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Aguilar, Hugo Marcelo;Abad-Franch, Fernando;Coura, José Rodrigues
通讯作者：
Coura, José Rodrigues
影响因子：
2.6
作者：
Darby, A. C.;Choi, J. -H.;Colbourne, J. K.
通讯作者：
Colbourne, J. K.
影响因子：
4.4
作者：
Ashelford, Kevin E.;Chuzhanova, Nadia A.;Weightman, Andrew J.
通讯作者：
Weightman, Andrew J.