Galectin-4 expression is down-regulated in response to autophagy during differentiation of rat trophoblast cells.
Galectin-4 expression is down-regulated in response to autophagy during differentiation of rat trophoblast cells.
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DOI：
10.1038/srep32248
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发表时间：
2016-08-30
影响因子：
4.6
通讯作者：
Shoji H
中科院分区：
文献类型：
--
作者：
Arikawa T;Liao S;Shimada H;Inoue T;Sakata-Haga H;Nakamura T;Hatta T;Shoji H
胎盘的发育和母体子宫内膜滋养细胞的侵袭建立了母胎界面，这对胚胎和胎儿的发育至关重要。本研究表明，在滋养层分化过程中，半乳糖凝集素-4 （Gal-4）的过度表达抑制了Rcho-1细胞（大鼠滋养层分化模型）的扩大，促进了细胞间的粘附，而滋养层特异性标记物和MMP-9活性不受影响。在大鼠胎盘中，自噬标志物微管相关蛋白1轻链3 α (LC3)蛋白在蜕膜母体侧高表达，而Gal-4表达较弱。体外实验显示，与对照细胞相比，被称为自噬抑制剂的3-甲基腺嘌呤（3-MA）和巴菲霉素A1降低了滋养层细胞特异性分化标志物的表达。此外，在Rcho-1细胞中，Gal-4的表达在分化过程中通常是下调的，但在自噬抑制剂的存在下，Gal-4的表达并没有减弱，这表明自噬是Gal-4表达的上游。我们在此描述了自噬通过调节Gal-4表达来调节滋养细胞分化以建立母胎界面的可能机制。
Placental development and trophoblast invasion of the maternal endometrium establish the maternal-fetal interface, which is critical for the developing embryo and fetus. Herein we show that overexpression of Galectin-4 (Gal-4) during trophoblast differentiation inhibited the enlargement of Rcho-1 cells (a model for rat trophoblast differentiation) and promoted cell-cell adhesion, whereas trophoblast specific markers and MMP-9 activity were not affected. In the rat placenta, microtubule associated protein 1 light chain 3 alpha (LC3) protein, an autophagy marker, is highly expressed on the maternal side of the decidua where Gal-4 expression is weak. In vitro assays showed that the expression of trophoblast-specific differentiation markers was reduced by 3-Methyladenine (3-MA) and Bafilomycin A1, known as autophagy inhibitors, compared to control cells. Furthermore, Gal-4 expression in Rcho-1 cells, which is normally down-regulated during differentiation, was not attenuated in the presence of autophagy inhibitors, suggesting that autophagy is upstream of Gal-4 expression. We herein describe a possible mechanism by which autophagy regulates trophoblast differentiation via regulation of Gal-4 expression in order to establish the maternal-fetal interface.
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影响因子：
5.7
作者：
Cai Z;Zeng Y;Xu B;Gao Y;Wang S;Zeng J;Chen L;Huang A;Liu X;Liu J
通讯作者：
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影响因子：
6.4
作者：
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通讯作者：
Rao, U. Subrahmanyeswara
影响因子：
4.9
作者：
Paclik, Daniela;Lohse, Katrin;Sturm, Andreas
通讯作者：
Sturm, Andreas
影响因子：
2.7
作者：
Ain, R;Canham, LN;Soares, MJ
通讯作者：
Soares, MJ
影响因子：
--
作者：
Maftouh M;Belo AI;Avan A;Funel N;Peters GJ;Giovannetti E;Van Die I
通讯作者：
Van Die I