Compressive force regulates ephrinB2 and EphB4 in osteoblasts and osteoclasts contributing to alveolar bone resorption during experimental tooth movement.
Compressive force regulates ephrinB2 and EphB4 in osteoblasts and osteoclasts contributing to alveolar bone resorption during experimental tooth movement.
复制标题
压力调节成骨细胞和破骨细胞中的 ephrinB2 和 EphB4，有助于实验性牙齿移动过程中的牙槽骨吸收
DOI：
10.4041/kjod.2014.44.6.320
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发表时间：
2014-11
影响因子：
1.9
通讯作者：
Sun H
中科院分区：
文献类型：
--
作者：
Hou J;Chen Y;Meng X;Shi C;Li C;Chen Y;Sun H
目的探讨ephrinB2在正畸牙移动过程中牙周组织改建中的作用及压力对成骨细胞和破骨细胞中EphB4和ephrinB2表达的影响。方法建立大鼠实验性牙齿移动模型，观察实验性牙齿移动过程中牙周组织的组织学变化及ephrinB2在牙周组织中的定位。RAW264.7细胞和ST 2细胞分别作为破骨细胞和成骨细胞的前体细胞，在体外受到压力。通过实时定量聚合酶链反应（PCR）检测EphB4和ephrinB2的基因表达，以及骨相关因子包括Runx2、Sp7、NFATc1和降钙素受体。结果实验组大鼠牙槽骨受压区的组织学观察显示，实验组大鼠牙槽骨受压区的破骨细胞生成活性增强，成骨细胞生成活性受到抑制。免疫组化显示ephrinB2在这些区域的破骨细胞中强表达。实时荧光定量PCR结果显示，压缩后RAW264. 7细胞中NFATc1、降钙素受体和ephrinB2的mRNA水平显著升高，而压缩后ST2细胞中ephrinB2、EphB4、Sp7和Runx2的mRNA水平显著降低。结论压力可调控成骨细胞和破骨细胞中EphB4和ephrinB2的表达，这可能与正畸牙移动过程中压力区牙槽骨吸收有关。
Objective To investigate the involvement of ephrinB2 in periodontal tissue remodeling in compression areas during orthodontic tooth movement and the effects of compressive force on EphB4 and ephrinB2 expression in osteoblasts and osteoclasts. Methods A rat model of experimental tooth movement was established to examine the histological changes and the localization of ephrinB2 in compressed periodontal tissues during experimental tooth movement. RAW264.7 cells and ST2 cells, used as precursor cells of osteoclasts and osteoblasts, respectively, were subjected to compressive force in vitro. The gene expression of EphB4 and ephrinB2, as well as bone-associated factors including Runx2, Sp7, NFATc1, and calcitonin receptor, were examined by quantitative real-time polymerase chain reaction (PCR). Results Histological examination of the compression areas of alveolar bone from experimental rats showed that osteoclastogenic activities were promoted while osteogenic activities were inhibited. Immunohistochemistry revealed that ephrinB2 was strongly expressed in osteoclasts in these areas. Quantitative real-time PCR showed that mRNA levels of NFATc1, calcitonin receptor, and ephrinB2 were increased significantly in compressed RAW264.7 cells, and the expression of ephrinB2, EphB4, Sp7, and Runx2 was decreased significantly in compressed ST2 cells. Conclusions Our results indicate that compressive force can regulate EphB4 and ephrinB2 expression in osteoblasts and osteoclasts, which might contribute to alveolar bone resorption in compression areas during orthodontic tooth movement.
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影响因子：
15.3
作者：
Asagiri, M;Sato, K;Takayanagi, H
通讯作者：
Takayanagi, H
影响因子：
29
作者：
Zhao, Chen;Irie, Naoko;Matsuo, Koichi
通讯作者：
Matsuo, Koichi
影响因子：
64.5
作者：
Nakashima, K;Zhou, X;de Crombrugghe, B
通讯作者：
de Crombrugghe, B
影响因子：
4.8
作者：
LEE, SK;GOLDRING, SR;LORENZO, JA
通讯作者：
LORENZO, JA
影响因子：
7.6
作者：
Kanzaki, H;Chiba, M;Mitani, H
通讯作者：
Mitani, H