AMIGO, a transmembrane protein implicated in axon tract development, defines a novel protein family with leucine-rich repeats.
AMIGO, a transmembrane protein implicated in axon tract development, defines a novel protein family with leucine-rich repeats.
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DOI：
10.1083/jcb.200209074
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发表时间：
2003-03-17
期刊：
影响因子：
--
通讯作者：
Rauvala H
中科院分区：
文献类型：
--
作者：
Kuja-Panula J;Kiiltomäki M;Yamashiro T;Rouhiainen A;Rauvala H
有序的差异显示确定了一个新的序列诱导的神经元的轴突促进蛋白terlipterin。我们将该基因命名为AMIGO，并克隆了两个与AMIGO同源的新基因（AMIGO 2和AMIGO 3）。这三个AMIGO共同形成编码I型跨膜蛋白的新基因家族，所述I型跨膜蛋白含有用于分泌的信号序列和跨膜结构域。AMIGO的推导的细胞外部分包含六个富含亮氨酸的重复序列（LRR），两侧是富含半胱氨酸的LRR NH 2-和COOH-末端结构域和一个靠近跨膜区的免疫球蛋白结构域。底物结合形式的重组AMIGO胞外域促进海马神经元中突出的神经突延伸，并且在溶液中，相同的AMIGO胞外域抑制神经突的成束。AMIGO家族成员之间存在嗜同性和嗜异性结合机制。我们的研究结果表明，AMIGO蛋白家族的成员是新的细胞粘附分子，其中AMIGO是特异性表达的纤维束的神经组织，并参与其形成。
Ordered differential display identified a novel sequence induced in neurons by the neurite-promoting protein amphoterin. We named this gene amphoterin-induced gene and ORF (AMIGO), and also cloned two other novel genes homologous to AMIGO (AMIGO2 and AMIGO3). Together, these three AMIGOs form a novel family of genes coding for type I transmembrane proteins which contain a signal sequence for secretion and a transmembrane domain. The deduced extracellular parts of the AMIGOs contain six leucine-rich repeats (LRRs) flanked by cysteine-rich LRR NH2- and COOH-terminal domains and by one immunoglobulin domain close to the transmembrane region. A substrate-bound form of the recombinant AMIGO ectodomain promoted prominent neurite extension in hippocampal neurons, and in solution, the same AMIGO ectodomain inhibited fasciculation of neurites. A homophilic and heterophilic binding mechanism is shown between the members of the AMIGO family. Our results suggest that the members of the AMIGO protein family are novel cell adhesion molecules among which AMIGO is specifically expressed on fiber tracts of neuronal tissues and participates in their formation.
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影响因子：
64.5
作者：
Brose, K;Bland, KS;Kidd, T
通讯作者：
Kidd, T
小鼠发育过程中破骨细胞谱系中 92-kD IV 型胶原酶（明胶酶 B）的高表达。
影响因子：
7.8
作者：
Reponen, P;Sahlberg, C;Munaut, C;Thesleff, I;Tryggvason, K
通讯作者：
Tryggvason, K
影响因子：
4.4
作者：
Nagasawa, A;Kubota, R;Shimizu, N
通讯作者：
Shimizu, N
DOI：
10.1006/bbrc.2001.5214
发表时间：
2001-07-20
影响因子：
3.1
作者：
Agarwala, KL;Ganesh, S;Yamakawa, K
通讯作者：
Yamakawa, K
影响因子：
7.5
作者：
Schachner, M
通讯作者：
Schachner, M