Effects of Wolbachia on rDNA-ITS2 variation and evolution in natural populations of Tetranychus urticae Koch

Effects of Wolbachia on rDNA-ITS2 variation and evolution in natural populations of Tetranychus urticae Koch
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沃尔巴克氏体对二点叶螨自然种群 rDNA-ITS2 变异和进化的影响

DOI:
10.11158/saa.17.1.9
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发表时间:
2012-02
影响因子:
1.2
通讯作者:
Yi-Zhong Yang
Yi-Zhong Yang
中科院分区:
农林科学3区
文献类型:
--
作者:
Hong-Hua Su;Xian-Ming Yang;Ming-Zhi Yu;Feng Jiang;Xiao-Yue Hong;Yi-Zhong Yang

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摘要本研究通过对二斑叶螨rDNA-ITS 2基因进行测序分析,研究了Wolbachia对四个种群二斑叶螨rDNA的影响。结果表明,四个群体的T.荨麻疹感染两株沃尔巴克氏体。它们是wUrtOri 1(HM 486515-HM 486517)和wUrtCon 1(HM 486518),分别属于B超群的Ori群和Con群。在ITS 2片段的645个位点中有一个突变位点。所有ITS 2基因序列可分为两种单倍型。个体ITS 2序列的多样性分析表明,感染Wolbachia并没有显著改变rDNA的多样性。ITS 2的中性检验表明,T.荨麻疹在进化过程中保持稳定,没有脱离哈代-温伯格平衡。
Abstract In this study, rDNA-ITS2 was sequenced and analyzed to investigate the effect of Wolbachia on the rDNA of four populations of Tetranychus urticae Koch. The result showed that all four populations of T. urticae were infected with two strains of Wolbachia. They are wUrtOri1 (HM486515–HM486517) and wUrtCon1 (HM486518), belonging to the Ori group and Con group of B supergroup, respectively. There was one mutation site among 645 sites for the ITS2 fragments. All the sequenced ITS2 were classified into two haplotypes. The diversity analysis of ITS2 sequences of individuals showed that infection by Wolbachia did not significantly change the diversity of rDNA. A neutrality test using ITS2 indicated that the population of T. urticae has remained stable during evolution and is not off the Hardy-Weinberg equilibrium.
DOI: 10.1098/rstb.2000.0716
发表时间: 2000-11-29
影响因子: 6.3
作者:
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