The function of follicular helper T cells is regulated by the strength of T cell antigen receptor binding.
The function of follicular helper T cells is regulated by the strength of T cell antigen receptor binding.
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滤泡辅助性T细胞（TFH细胞）如何分化以调节B细胞免疫对于有效的蛋白质疫苗接种至关重要。在这里，我们定义了三个转录因子T-bet表达抗原特异性效应辅助T细胞亚群，具有可区分的功能，迁移特性和体内发育编程。转录阻遏物Blimp-1的表达将T区“淋巴样”效应辅助T细胞（CD 62 LhiCCR 7 hi）与表达转录阻遏物Bcl-6的CXCR 5lo“迁移的”效应辅助T细胞和CXCR 5 hi“驻留的”TFH细胞（CD 62 LloCCR 7 lo）区分开。然后，我们通过过继转移和完整的多克隆反应表明，辅助T细胞与肽-主要组织相容性复合物II类的最高特异性结合和最受限制的T细胞抗原受体连接多样性“优先”发展成抗原特异性效应TFH隔室。我们的研究表明，在抗原特异性机制中，T细胞抗原受体的结合强度差异的中心功能，“程序”专门的效应TFH功能在体内。
How follicular helper T cells (TFH cells) differentiate to regulate B cell immunity is critical for effective protein vaccination. Here we define three transcription factor T-bet-expressing antigen-specific effector helper T cell subsets with distinguishable function, migratory properties and developmental programming in vivo. Expression of the transcriptional repressor Blimp-1 distinguished T zone ‘lymphoid’ effector helper T cells (CD62LhiCCR7hi) from CXCR5lo ‘emigrant’ effector helper T cells and CXCR5hi ‘resident’ TFH cells expressing the transcriptional repressor Bcl-6 (CD62LloCCR7lo). We then show by adoptive transfer and intact polyclonal responses that helper T cells with the highest specific binding of peptide-major histocompatibility complex class II and the most restricted T cell antigen receptor junctional diversity ‘preferentially’ developed into the antigen-specific effector TFH compartment. Our studies demonstrate a central function for differences in the binding strength of the T cell antigen receptor in the antigen-specific mechanisms that ‘program’ specialized effector TFH function in vivo.
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DOI：
10.1084/jem.182.5.1591
发表时间：
1995-11-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Constant S;Pfeiffer C;Woodard A;Pasqualini T;Bottomly K
通讯作者：
Bottomly K
DOI：
10.1084/jem.191.12.2065
发表时间：
2000-06-19
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Blander JM;Sant'Angelo DB;Bottomly K;Janeway CA Jr
通讯作者：
Janeway CA Jr
影响因子：
30.5
作者：
Henrickson, Sarah E.;Mempel, Thorsten R.;von Andrian, Ulrich H.
通讯作者：
von Andrian, Ulrich H.
影响因子：
32.4
作者：
Celli, Susanna;Lemaitre, Fabrice;Bousso, Philippe
通讯作者：
Bousso, Philippe
影响因子：
32.4
作者：
Malherbe, Laurent;Mark, Linda;McHeyzer-Williams, Michael G.
通讯作者：
McHeyzer-Williams, Michael G.