Macrophage Populations in Visceral Adipose Tissue from Pregnant Women: Potential Role of Obesity in Maternal Inflammation.
Macrophage Populations in Visceral Adipose Tissue from Pregnant Women: Potential Role of Obesity in Maternal Inflammation.
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DOI：
10.3390/ijms19041074
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发表时间：
2018-04-04
影响因子：
5.6
通讯作者：
Estrada-Gutierrez G
中科院分区：
文献类型：
--
作者：
Bravo-Flores E;Mancilla-Herrera I;Espino Y Sosa S;Ortiz-Ramirez M;Flores-Rueda V;Ibargüengoitia-Ochoa F;Ibañez CA;Zambrano E;Solis-Paredes M;Perichart-Perera O;Sanchez-Martinez M;Medina-Bastidas D;Reyes-Muñoz E;Estrada-Gutierrez G
肥胖与脂肪组织巨噬细胞（ATMs）的炎症变化、积累和表型极化有关。肥胖孕妇有脂肪组织组成的改变，但巨噬细胞群体的详细描述是不可用的。在这项研究中，我们对正常、超重和肥胖孕妇的内脏脂肪组织（VAT）中的巨噬细胞群体进行了表征。利用流式细胞术对VAT活组织巨噬细胞进行免疫分型，分别使用CD45和CD14作为造血和单核细胞谱系的标记，同时使用HLA-DR、CD11c、CD163和CD206作为促炎和抗炎标记。光镜下观察脂肪细胞的数量和大小。结果表明，妊娠期体重超重和肥胖的孕妇存在脂肪细胞肥大。在VAT中发现了两种不同的巨噬细胞群体：募集的巨噬细胞（CD45+CD14+）和缺乏CD45的新群体，这被认为是一个常驻巨噬细胞亚群（CD45−CD14+）。HLA - DRlow/ -巨噬细胞数量与身体质量指数（BMI）呈负相关。肥胖女性的常驻和招募巨噬细胞表达更高的CD206水平。肥胖女性的HLA-DR+巨噬细胞中CD11c表达较高。CD206和CD11c标记物与BMI有很强的相关性。我们的研究结果表明，妊娠期超重和肥胖与脂肪细胞肥大和VAT中的特定atm种群有关。
Obesity is associated with inflammatory changes and accumulation and phenotype polarization of adipose tissue macrophages (ATMs). Obese pregnant women have alterations in adipose tissue composition, but a detailed description of macrophage population is not available. In this study, we characterized macrophage populations in visceral adipose tissue (VAT) from pregnant women with normal, overweight, and obese pregestational weight. Immunophenotyping of macrophages from VAT biopsies was performed by flow cytometry using CD45 and CD14 as markers of hematopoietic and monocyte linage, respectively, while HLA-DR, CD11c, CD163, and CD206 were used as pro- and anti-inflammatory markers. Adipocyte number and size were evaluated by light microscopy. The results show that pregnant women that were overweight and obese during the pregestational period had adipocyte hypertrophy. Two different macrophage populations in VAT were identified: recruited macrophages (CD45+CD14+), and a novel population lacking CD45, which was considered to be a resident macrophages subset (CD45−CD14+). The number of resident HLA−DRlow/− macrophages showed a negative correlation with body mass index (BMI). Both resident and recruited macrophages from obese women expressed higher CD206 levels. CD11c expression was higher in resident HLA-DR+ macrophages from obese women. A strong correlation between CD206 and CD11c markers and BMI was observed. Our findings show that being overweight and obese in the pregestational period is associated with adipocyte hypertrophy and specific ATMs populations in VAT.
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影响因子：
6.9
作者：
Haghiac, Maricela;Vora, Neeta L.;Basu, Subhabrata;Johnson, Kirby L.;Presley, Larraine;Bianchi, Diana W.;Hauguel-de Mouzon, Sylvie
通讯作者：
Hauguel-de Mouzon, Sylvie
影响因子：
3.8
作者：
Huh JY;Park YJ;Ham M;Kim JB
通讯作者：
Kim JB
DOI：
10.1530/joe-13-0339
发表时间：
2014-02
期刊：
The Journal of endocrinology
影响因子：
--
作者：
Cao H
通讯作者：
Cao H
DOI：
10.1084/jem.20120340
发表时间：
2012-06-04
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hoeffel G;Wang Y;Greter M;See P;Teo P;Malleret B;Leboeuf M;Low D;Oller G;Almeida F;Choy SH;Grisotto M;Renia L;Conway SJ;Stanley ER;Chan JK;Ng LG;Samokhvalov IM;Merad M;Ginhoux F
通讯作者：
Ginhoux F
巨噬细胞的局部扩散有助于与肥胖相关的脂肪组织炎症。
影响因子：
29
作者：
Amano SU;Cohen JL;Vangala P;Tencerova M;Nicoloro SM;Yawe JC;Shen Y;Czech MP;Aouadi M
通讯作者：
Aouadi M