Partial resistance to clubroot in Arabidopsis is based on changes in the host primary metabolism and targeted cell division and expansion capacity.
Partial resistance to clubroot in Arabidopsis is based on changes in the host primary metabolism and targeted cell division and expansion capacity.
复制标题
DOI：
10.1007/s10142-013-0312-9
复制
发表时间：
2013-06
影响因子：
2.9
通讯作者：
Manzanares-Dauleux, Maria J.
中科院分区：
文献类型：
--
作者：
Jubault, Melanie;Lariagon, Christine;Taconnat, Ludivine;Renou, Jean-Pierre;Gravot, Antoine;Delourme, Regine;Manzanares-Dauleux, Maria J.
迄今为止，对抗病分子基础的研究主要集中在定性抗性上。然而，对数量抗性机制的解读将有助于深入了解定性和定量抗性之间的关系，并指导利用这两种类型的抗性来培育耐久抗性品种。使用CATMA全基因组微阵列的功能基因组学方法，用于检测拟南芥-芸苔plasmodiophora brassicae病理系统中与部分定量抗性相关的基因表达变化。在全株水平上监测了部分抗性应答过程中转录物丰度的时间过程，并利用同一寄主基因型直接比较了部分抗性应答和部分敏感性应答。揭示了一个日益复杂的宿主反应，以及根据所使用的分离物，油菜卟啉菌感染对拟南芥基因转录的差异影响。在转录组学水平上，我们观察到病原菌的代谢转移被减少或延迟，经典的植物防御反应被诱导得更早和/或更强烈，细胞的扩大和增殖在部分数量抗性反应中被积极抑制。本文的在线版本（doi:10.1007/s10142-013-0312-9）包含补充材料，仅供授权用户使用。
To date, studies of the molecular basis of disease resistance mainly focused on qualitative resistance. However, deciphering mechanisms underlying quantitative resistance could lead to insights into the relationship between qualitative and quantitative resistance and guide the utilization of these two types of resistance to produce durably resistant cultivars. A functional genomics approach, using the CATMA whole-genome microarray, was used to detect changes in gene expression associated with partial quantitative resistance in the Arabidopsis thaliana–Plasmodiophora brassicae pathosystem. The time course of transcript abundance during partial clubroot resistance response was monitored at the whole plant level, and direct comparisons between partial resistance and susceptibility responses were made using the same host genotype. An increasingly complex host response was revealed, as was the differential influence of P. brassicae infection on the transcription of Arabidopsis genes according to the isolate used. We observed, at the transcriptomic level, that metabolic diversion by the pathogen was reduced or delayed, classical plant defense responses were induced earlier and/or more strongly, and cell enlargement and proliferation were actively inhibited in the partial quantitative resistance response compared to the susceptible one. The online version of this article (doi:10.1007/s10142-013-0312-9) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
7.4
作者：
Allemeersch, J;Durinck, S;Kuiper, MTR
通讯作者：
Kuiper, MTR
影响因子：
3
作者：
Agarwal, Arati;Kaul, Vijay;Cahill, David M.
通讯作者：
Cahill, David M.
影响因子：
3.5
作者：
Devos, Sylvie;Laukens, Kris;Prinsen, Els
通讯作者：
Prinsen, Els
影响因子：
11.6
作者：
Deeken, Rosalia;Engelmann, Julia C.;Hedrich, Rainer
通讯作者：
Hedrich, Rainer
影响因子：
3.5
作者：
Huibers, Robin P.;de Jong, Mark;Van den Ackerveken, Guido
通讯作者：
Van den Ackerveken, Guido