Mesenchymal stem cells and extracellular matrix scaffold promote muscle regeneration by synergistically regulating macrophage polarization toward the M2 phenotype.
Mesenchymal stem cells and extracellular matrix scaffold promote muscle regeneration by synergistically regulating macrophage polarization toward the M2 phenotype.
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间充质干细胞和细胞外基质支架通过协同调节巨噬细胞极化向 M2 表型促进肌肉再生
DOI：
10.1186/s13287-018-0821-5
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发表时间：
2018-04-03
影响因子：
7.5
通讯作者：
Jin Y
中科院分区：
文献类型：
--
作者：
Qiu X;Liu S;Zhang H;Zhu B;Su Y;Zheng C;Tian R;Wang M;Kuang H;Zhao X;Jin Y
骨骼肌在人体的生理活动中起着重要的作用，但对于严重外伤或肿瘤切除引起的体积性肌肉损失（VML），目前还没有有效的治疗方法。最近的研究表明，使用含有间充质干细胞（MSCs）和脱细胞外基质（ECM）支架的化合物的组织工程策略对VML损伤产生了显着的再生作用，但其潜在机制尚不完全清楚。测定了人脐带间充质干细胞的增殖能力和多分化能力。我们构建了一种含有MSCs和脱细胞ECM支架的化合物，用于VML模型的组织再生。我们发现MSCs和脱细胞ECM支架在促进骨骼肌组织再生方面具有协同作用。有趣的是，MSCs和去细胞化的ECM支架都能促进巨噬细胞向M2表型极化，抑制巨噬细胞向M1表型极化，这被广泛认为是组织再生的重要促进因素。更重要的是，MSCs和去细胞化ECM支架对巨噬细胞向M2表型极化产生协同促进作用，而不仅仅是加性作用。我们的发现揭示了一个以前未知的机制，即MSCs和去细胞化的ECM支架通过协同调节巨噬细胞极化来促进组织再生。本文的在线版本（10.1186/s13287-018-0821-5）包含补充内容，仅供授权用户使用。
Skeletal muscle plays an important role in the body’s physiology but there are still no effective treatments for volumetric muscle loss (VML) resulting from severe traumatic injury or tumor excision. Recent studies show that a tissue engineering strategy using a compound containing mesenchymal stem cells (MSCs) and decellularized extracellular matrix (ECM) scaffold generates significant regenerative effects on VML injury, but the underlying mechanisms are not fully understood. The characteristics of human umbilical cord MSCs, including multiplication capacity and multidifferentiation ability, were determined. We constructed a compound containing MSCs and decellularized ECM scaffold which was used for tissue regeneration in a VML model. We found that MSCs and decellularized ECM scaffold generated synergistic effects on promoting skeletal muscle tissue regeneration. Interestingly, both MSCs and decellularized ECM scaffold could promote macrophage polarization toward the M2 phenotype and suppress macrophage polarization toward the M1 phenotype, which is widely regarded as an important promoting factor in tissue regeneration. More importantly, MSCs and decellularized ECM scaffold generate synergistic promoting effects on macrophage polarization toward the M2 phenotype, not just an additive effect. Our findings uncover a previously unknown mechanism that MSCs and decellularized ECM scaffold promote tissue regeneration via collaboratively regulating macrophage polarization. The online version of this article (10.1186/s13287-018-0821-5) contains supplementary material, which is available to authorized users.
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影响因子：
3.8
作者：
Duffy RM;Sun Y;Feinberg AW
通讯作者：
Feinberg AW
影响因子：
14
作者：
Ham, Onju;Song, Byeong-Wook;Hwang, Ki-Chul
通讯作者：
Hwang, Ki-Chul
影响因子：
--
作者：
De Coppi, Paolo;Bellini, Silvia;Paolo Parnigotto, Pier
通讯作者：
Paolo Parnigotto, Pier
DOI：
10.5435/00124635-201102001-00007
发表时间：
2011-01-01
影响因子：
3.2
作者：
Grogan, Brian F.;Hsu, Joseph R.
通讯作者：
Hsu, Joseph R.
影响因子：
2.6
作者：
Fuoco C;Petrilli LL;Cannata S;Gargioli C
通讯作者：
Gargioli C