Supramolecular Membrane Organization
Supramolecular Membrane Organization
复制标题
超分子膜组织
DOI：
10.1007/978-94-011-0227-8_6
复制
发表时间：
1994
期刊：
影响因子：
3.5
通讯作者：
E. Gantt
中科院分区：
文献类型：
--
作者：
E. Gantt
在蓝藻中，外膜、质膜和类囊体膜代表三种结构和功能不同的膜。类囊体膜研究的共同主题表明，主要的功能成分，包括光系统I、光系统II、细胞色素b6f、NADH脱氢酶、细胞色素氧化酶和ATP合成酶，以多亚基复合物的形式存在于膜内。这些完整的膜复合物穿过4-5纳米的脂质双分子层，并延伸到基质和管腔空间，从而使膜厚度增加了一倍。原位细胞色素b6f、细胞色素氧化酶和光系统II优先以二聚体形式出现，而光系统I优先以三聚体形式出现。测定每个类囊体区域的超分子复合物的密度以及它们之间的空间关系可以为了解膜的功能提供有用的信息。计算表明，至少50%的膜内面积被光系统I和II、细胞色素b6f和ATP合酶的CF0单独占据。膜的地形图可以用金抗体标记进行，分辨率约为15-20纳米。对外膜、质膜的配合物以及肽聚糖层的组成和影响的研究仍然很少，需要进一步拓展。蓝藻膜结构的独特性不断涌现。与其他真细菌相比，外壁层结构的差异已被注意到；然而，在原绿藻和真核藻类中也发现了必需的类囊体特征。这些特征强调蓝藻和原绿藻应该被认为是真细菌的一个独特的分组。
In cyanobacteria the outer membrane, plasma membrane and thylakoid membrane represent three structurally and functionally distinct membranes. Common themes are emerging from studies of thylakoid membranes which show that the major functional components, including Photosystem I, Photosystem II, cytochrome b 6 f, NADH dehydrogenase, cytochrome oxidase, and ATP synthase, exist as multisubunit complexes within the membrane. These integral membrane complexes traverse the 4–5 nm lipid bilayer and extend into the stromal and lumenal spaces-thus virtually doubling the membrane thickness. In situ it appears that cytochrome b 6 f, cytochrome oxidase, and Photosystem II preferentially occur as dimers and Photosystem I as trimers. Determination of the density of the supramolecular complexes per thylakoid area and the spatial relationships among them can provide useful insight into membrane functional. Calculations suggest that at least 50% of the intramembrane area is occupied by photosystems I and II, cytochrome b 6 f, and CF0 of ATP synthase alone. Topographic mapping of the membranes can be pursued with gold-antibody labeling with a resolution of approximately 15–20 nm. Studies on complexes of the outer membrane, the plasma membrane, and the composition and influence of the peptidoglycan layer are still scarce and should be extended. The uniqueness of cyanobacterial membrane structure continues to emerge. Differences in the structure of the outer wall layers have been noted when compared to other eubacteria; however, essential thylakoid features are also found in prochlorophytes and eucaryotic algae oxygenic plants. These features emphasize that cyanobacteria and prochlorophytes should be considered a distinctive grouping of the eubacteria.
DOI：
10.1111/j.1432-1033.1992.tb16857.x
发表时间：
1992-05-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
GLAUSER, M;BRYANT, DA;ZUBER, H
通讯作者：
ZUBER, H
细菌中的转运蛋白：其设计中的常见主题。
DOI：
10.1126/science.1279804
发表时间：
1992
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Nikaido,H;SaierJr,MH
通讯作者：
SaierJr,MH
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Pedersen,PL;Amzel,LM
通讯作者：
Amzel,LM