Analysis of the genetic diversity and mRNA expression level in porcine reproductive and respiratory syndrome virus vaccinated pigs that developed short or long viremias after challenge.

Analysis of the genetic diversity and mRNA expression level in porcine reproductive and respiratory syndrome virus vaccinated pigs that developed short or long viremias after challenge.
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DOI:
10.1186/s13567-018-0514-1
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发表时间:
2018-02-15
影响因子:
4.4
通讯作者:
Darwich L
Darwich L
中科院分区:
农林科学2区
文献类型:
--
作者:
Cortey M;Arocena G;Ait-Ali T;Vidal A;Li Y;Martín-Valls G;Wilson AD;Archibald AL;Mateu E;Darwich L

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猪繁殖与呼吸综合征病毒 (PRRSv) 感染会改变宿主的细胞和体液免疫反应。针对 PRRSv 的免疫力是多基因的,并且因个体而异。本研究的目的是比较两组接种疫苗的猪之间参与免疫反应的分子的编码基因,这些猪在PRRSv攻击后经历了短期或长期的病毒血症期。这些分析包括四个 SLA I 类、两个 II 类等位基因组和 CD163 的测序,以及通过定量实时 qRT-PCR 分析外周血单核细胞中 TLR2、TLR3、TLR4、TLR7、TLR8 和 TLR9 mRNA 及其他分子的组成型表达。
Porcine reproductive and respiratory syndrome virus (PRRSv) infection alters the host’s cellular and humoral immune response. Immunity against PRRSv is multigenic and vary between individuals. The aim of the present study was to compare several genes that encode for molecules involved in the immune response between two groups of vaccinated pigs that experienced short or long viremic periods after PRRSv challenge. These analyses include the sequencing of four SLA Class I, two Class II allele groups, and CD163, plus the analysis by quantitative realtime qRT-PCR of the constitutive expression of TLR2, TLR3, TLR4, TLR7, TLR8 and TLR9 mRNA and other molecules in peripheral blood mononuclear cells.
全基因组的关联和基因组预测,用于宿主对猪生殖和呼吸道综合征病毒感染的反应。
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影响因子: --
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