Ovarian hormone-induced reorganization of oxytocin-labeled dendrites and synapses lateral to the hypothalamic ventromedial nucleus in female rats.
Ovarian hormone-induced reorganization of oxytocin-labeled dendrites and synapses lateral to the hypothalamic ventromedial nucleus in female rats.
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DOI：
10.1002/cne.22470
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发表时间：
2010-11-15
影响因子：
2.5
通讯作者：
Flanagan-Cato, Loretta M.
中科院分区：
文献类型：
--
作者：
Griffin, Gerald D.;Ferri-Kolwicz, Sarah L.;Reyes, Beverly A. S.;Van Bockstaele, Elisabeth J.;Flanagan-Cato, Loretta M.
关键词：
中枢催产素(OT)调节许多社会行为，包括雌性大鼠性接受能力，被量化为交配姿势，称为前凸。行为药理学实验证明，下丘脑腹内侧核(VMH)的OT作用调节了前凸反应的表达。然而，OT在这一脑区的亚细胞定位尚不清楚。我们用免疫电子显微镜验证了卵巢激素在VMH外侧纤维复合体中重组OT标记的突触前或突触后成分的假说。OT免疫标记出现在轴突周围，发现小而清晰的突触小泡，并用突触前标记突触素和囊泡谷氨酸转运蛋白2进行双重标记。在树突中也观察到OT免疫反应，树突特异性标记微管相关蛋白2的双重标记证实了OT免疫反应。卵巢激素不改变轴突的密度；然而，雌二醇处理使树突样密度减少了34%。当在雌二醇之后给予黄体酮时，这种作用被逆转。雌激素治疗的效果仅限于缺乏OT免疫染色的树突状细胞；在雌激素治疗期间，OT标记的树突状细胞的密度保持不变。随着雌二醇诱导的非OT标记的树突状轮廓的退出，其余的OT标记的树突状轮廓经历了它们的突触接触的增加。因此，模拟4天大鼠发情周期的激素处理在VMH外侧的纤维丛中激发了树突神经的化学编码重组，这可能是OT对生殖行为产生激素特异性效应的基础。
Central oxytocin (OT) modulates many social behaviors, including female rat sexual receptivity, quantified as the copulatory stance known as lordosis. The expression of the lordosis response is modulated by OT action in the ventromedial nucleus of the hypothalamus (VMH), as demonstrated by behavioral pharmacology experiments. However, the subcellular localization of OT in this brain region has been unclear. We tested the hypothesis that ovarian hormones reorganize OT-labeled pre- or postsynaptic elements in the fiber complex lateral to the VMH by using immunoelectron microscopy. OT immunolabeling occurred in axonal boutons identified by the presence of small, clear synaptic vesicles and double labeling with the presynaptic markers synaptophysin and vesicular glutamate transporter 2. OT immunoreactivity also was observed in dendritic profiles, verified with double labeling for the dendrite-specific marker microtubule-associated protein 2. Ovarian hormones did not alter the density of axonal boutons; however, estradiol treatment reduced the density of dendritic profiles by 34%. This effect was reversed when progesterone was given subsequent to estradiol. The effect of estradiol treatment was specific to dendrites that lacked OT immunostaining; the density of OT-labeled dendritic profiles remained constant during estradiol treatment. With the estradiol-induced exit of non-OT-labeled dendritic profiles, the remaining OT-labeled dendritic profiles experienced an increase in their number of synaptic contacts. Thus, hormone treatments that mimic the 4- day rat estrous cycle provoke a chemically coded reorganization of dendrite innervation in the fiber plexus lateral to the VMH that may underlie the hormone-specific effect of OT on reproductive behavior.
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影响因子：
4.8
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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影响因子：
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作者：
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通讯作者：
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作者：
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