CPK28-NLP7 module integrates cold-induced Ca(2+) signal and transcriptional reprogramming in Arabidopsis.
CPK28-NLP7 module integrates cold-induced Ca(2+) signal and transcriptional reprogramming in Arabidopsis.
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CPK 28-NLP 7模块整合拟南芥冷诱导Ca（2+）信号和转录重编程
DOI：
10.1126/sciadv.abn7901
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发表时间：
2022-07
期刊：
影响因子：
13.6
通讯作者：
中科院分区：
文献类型：
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作者：
暴露于寒冷触发细胞溶质钙（Ca 2+）的峰值，这通常导致植物中的转录重编程。然而，这种Ca 2+信号如何被感知并传递到下游冷信号通路仍然未知。在这里，我们表明，钙依赖性蛋白激酶28（CPK 28）启动磷酸化级联反应，以指定转录重编程下游冷诱导的Ca 2+信号。冷休克后10秒内，质膜（PM）定位的CPK 28以Ca 2+依赖性方式迅速激活。然后CPK 28磷酸化并促进NIN样蛋白7（NLP 7）的核转位，NLP 7是一种转录因子，其指定响应于Ca 2+的冷响应基因组的转录重编程，从而正向调节植物对冷胁迫的响应。这项研究阐明了一个以前未确定的机制，通过该机制，CPK 28-NLP 7调节模块整合了冷诱发的Ca 2+信号和转录组，从而揭示了冷信号从PM到核的快速感知和转导的关键策略。CPK 28-NLP 7在植物对冷胁迫的应答中感知和解码冷诱导的Ca 2+信号并导致转录重编程。
Exposure to cold triggers a spike in cytosolic calcium (Ca2+) that often leads to transcriptional reprogramming in plants. However, how this Ca2+ signal is perceived and relayed to the downstream cold signaling pathway remains unknown. Here, we show that the CALCIUM-DEPENDENT PROTEIN KINASE 28 (CPK28) initiates a phosphorylation cascade to specify transcriptional reprogramming downstream of cold-induced Ca2+ signal. Plasma membrane (PM)–localized CPK28 is activated rapidly upon cold shock within 10 seconds in a Ca2+-dependent manner. CPK28 then phosphorylates and promotes the nuclear translocation of NIN-LIKE PROTEIN 7 (NLP7), a transcription factor that specifies the transcriptional reprogramming of cold-responsive gene sets in response to Ca2+, thereby positively regulating plant response to cold stress. This study elucidates a previously unidentified mechanism by which the CPK28-NLP7 regulatory module integrates cold-evoked Ca2+ signal and transcriptome and thus uncovers a key strategy for the rapid perception and transduction of cold signals from the PM to the nucleus. CPK28-NLP7 senses and decodes cold-evoked Ca2+ signal and leads to transcriptional reprogramming in plant response to cold stress.
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影响因子：
20.5
作者：
Boudsocq, Marie;Sheen, Jen
通讯作者：
Sheen, Jen
DOI：
10.1073/pnas.1422446112
发表时间：
2015-05-19
影响因子：
11.1
作者：
Cybulski, Larisa Estefania;Ballering, Joost;Killian, J. Antoinette
通讯作者：
Killian, J. Antoinette
钙依赖性蛋白激酶 CPK28 以蛋氨酸腺苷转移酶为目标，被 26S 蛋白酶体降解，并影响拟南芥中的乙烯生物合成和木质素沉积
影响因子：
7.2
作者：
Jin, Yu;Ye, Nenghui;Zhang, Jianhua
通讯作者：
Zhang, Jianhua
影响因子：
4.9
作者：
Barajas-Lopez, Juan de Dios;Tiwari, Arjun;Fujii, Hiroaki
通讯作者：
Fujii, Hiroaki
影响因子：
64.8
作者：
通讯作者：
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