Melanocortin-4 receptor expression in a vago-vagal circuitry involved in postprandial functions.
Melanocortin-4 receptor expression in a vago-vagal circuitry involved in postprandial functions.
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DOI：
10.1002/cne.22221
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Elmquist J
中科院分区：
文献类型：
--
作者：
Gautron L;Lee C;Funahashi H;Friedman J;Lee S;Elmquist J
迷走神经传入通过在大脑和源自肠道的餐后信号之间建立联系来调节能量平衡。在本研究中，我们研究了黑素皮质素-4受体(MC4R)在迷走神经感觉传入细胞体所在的结状神经节中的表达。利用在MC4R启动子控制下表达绿色荧光蛋白(GFP)的小鼠，我们发现GFP在大约三分之一的结状神经节神经元中表达。用免疫组织化学结合原位杂交的方法，我们还发现有20%的绿色荧光蛋白阳性神经元共表达∼受体A。此外，我们还发现，绿色荧光蛋白通过迷走感觉传入和运动传出传递到周围组织，这使得我们能够评估Mc4R-绿色荧光蛋白神经元支配的部位。共同表达胆碱乙酰转移酶的GFP阳性传出，特异性地终止于肝动脉以及胃和十二指肠的肌间神经丛。相反，不表达胆碱能或交感神经标记物的GFP阳性传入终止于十二指肠粘膜下神经丛和粘膜。逆行追踪实验证实了位于结状节的GFP阳性神经元对十二指肠的神经支配。我们的发现支持迷走神经传入的MC4R信号可能调节对饱腹感信号敏感的纤维的活动，迷走神经传出的MC4R信号可能有助于肝脏和胃肠道的控制。
Vagal afferents regulate energy balance by providing a link between the brain and postprandial signals originating from the gut. In the current study, we investigated melanocortin-4 receptor (MC4R) expression in the nodose ganglion, where the cell bodies of vagal sensory afferents reside. Using a line of mice expressing Green Fluorescent Protein (GFP) under the control of the MC4R promoter, we found GFP expression in approximately one third of nodose ganglion neurons. Using immunohistochemistry combined with in situ hybridization, we also demonstrated that ∼20% of GFP-positive neurons coexpressed cholecystokinin receptor A. In addition, we found that the GFP is transported to peripheral tissues by both vagal sensory afferents and motor efferents, which allowed us to assess the sites innervated by MC4R-GFP neurons. GFP-positive efferents that co-expressed choline acetyltransferase specifically terminated in the hepatic artery and the myenteric plexus of the stomach and duodenum. In contrast, GFP-positive afferents that did not express cholinergic or sympathetic markers terminated in the submucosal plexus and mucosa of the duodenum. Retrograde tracing experiments confirmed the innervation of the duodenum by GFP-positive neurons located in the nodose ganglion. Our findings support the hypothesis that MC4R signaling in vagal afferents may modulate the activity of fibers sensitive to satiety signals such as cholecystokinin, and that MC4R signaling in vagal efferents may contribute to the control of the liver and gastrointestinal tract.
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影响因子：
64.8
作者：
Fan, W;Boston, BA;Cone, RD
通讯作者：
Cone, RD
影响因子：
2.5
作者：
BERTHOUD, HR;JEDRZEJEWSKA, A;POWLEY, TL
通讯作者：
POWLEY, TL
DOI：
10.1002/ar.a.20088
发表时间：
2004-09-01
期刊：
ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY
影响因子：
--
作者：
Berthoud, HR
通讯作者：
Berthoud, HR
DOI：
10.1097/med.0b013e3280122850
发表时间：
2007-02-01
期刊：
Current opinion in endocrinology, diabetes, and obesity
影响因子：
--
作者：
Chandra, Rashmi;Liddle, Rodger A
通讯作者：
Liddle, Rodger A
影响因子：
3.6
作者：
Chiocchetti, R;Grandis, A;Furness, JB
通讯作者：
Furness, JB