The adult microbiome of healthy and otitis patients: Definition of the core healthy and diseased ear microbiomes.
The adult microbiome of healthy and otitis patients: Definition of the core healthy and diseased ear microbiomes.
复制标题
DOI：
10.1371/journal.pone.0262806
复制
发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Dawson TL Jr
中科院分区：
文献类型：
--
作者：
Burton M;Krumbeck JA;Wu G;Tang S;Prem A;Gupta AK;Dawson TL Jr
中耳炎（OM）和外耳炎（OE）是疼痛的、复发性耳部疾病。由于大多数耳炎出版物关注儿童耳朵的细菌含量，因此仍然缺乏关于成人耳朵微生物组的信息，包括细菌和真菌。这项研究使用基因间转录间隔区（ITS）和16S-rDNA测序比较了健康成人与受OE和OM影响的成人的外耳微生物组。成年人耳芯微生物组由原核生物痤疮皮肤杆菌和真核生物阿鲁纳洛克马拉色菌、M. globosa和M.限制。健康耳真菌生物群以马拉色菌属为主，可分为两组，一组以马拉色菌属为主; arunalokei，另一个是M.限制。微生物组多样性和生物量在健康和患病耳朵之间存在显著差异，分析表明马拉色菌属和C.粉刺。健康的耳芯微生物组包括细菌头葡萄球菌和S. capitis/captured，而患病的耳芯由已知的细菌和真菌病原体组成，包括曲霉属，念珠菌属，铜绿假单胞菌、S.金黄色葡萄球菌和杰氏棒状杆菌。所提供的数据突出了早期发现中耳炎病因的必要性，以指导更适当、有效的治疗。这将改善患者的预后，并促进改善抗菌管理。
Otitis media (OM) and externa (OE) are painful, recurrent ear conditions. As most otitis publications focus on the bacterial content of childhood ears, there remains a dearth of information regarding the adult ear microbiome including both bacteria and fungi. This study compares the outer ear microbiome of healthy adults to adults affected by OE and OM using both intergenic-transcribed-spacer (ITS) and 16S-rDNA sequencing. The adult ear core microbiome consists of the prokaryote Cutibacterium acnes and the eukaryotic Malassezia arunalokei, M. globosa, and M. restricta. The healthy ear mycobiome is dominated by Malassezia and can be divided into two groups, one dominated by M. arunalokei, the other by M. restricta. Microbiome diversity and biomass varied significantly between healthy and diseased ears, and analyses reveal the presence of a potential mutualistic, protective effect of Malassezia species and C. acnes. The healthy ear core microbiome includes the bacteria Staphylococcus capitis and S. capitis/caprae, while the diseased ear core is composed of known bacterial and fungal pathogens including Aspergillus sp., Candida sp., Pseudomonas aeruginosa, S. aureus, and Corynebacterium jeikeium. The data presented highlight the need for early detection of the cause of otitis to direct more appropriate, efficient treatments. This will improve patient outcomes and promote improved antimicrobial stewardship.
登录
查看更多内容
影响因子：
9.7
作者：
Smets, Wenke;Leff, Jonathan W.;Fierer, Noah
通讯作者：
Fierer, Noah
DOI：
10.1016/b978-1-4557-4801-3.00062-x
发表时间：
2014-10-31
期刊：
Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases
影响因子：
--
作者：
Klein JO
通讯作者：
Klein JO
影响因子：
2.9
作者：
Han, Anne;Zenilman, Jonathan M.;Lazarus, Gerald S.
通讯作者：
Lazarus, Gerald S.
影响因子：
16.6
作者：
Segata, Nicola;Boernigen, Daniela;Morgan, Xochitl C.;Huttenhower, Curtis
通讯作者：
Huttenhower, Curtis
DOI：
10.1016/j.cmi.2017.10.013
发表时间：
2018-04
期刊：
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
影响因子：
--
作者：
Besser J;Carleton HA;Gerner-Smidt P;Lindsey RL;Trees E
通讯作者：
Trees E