Plasma specific miRNAs as predictive biomarkers for diagnosis and prognosis of glioma.
Plasma specific miRNAs as predictive biomarkers for diagnosis and prognosis of glioma.
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血浆特异性 miRNA 作为神经胶质瘤诊断和预后的预测生物标志物
DOI：
10.1186/1756-9966-31-97
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发表时间：
2012-11-22
期刊：
影响因子：
--
通讯作者：
Xie K
中科院分区：
文献类型：
--
作者：
Wang Q;Li P;Li A;Jiang W;Wang H;Wang J;Xie K
多形性胶质母细胞瘤(GBM)是一种恶性程度高、预后不良的脑肿瘤。MicroRNAs(MiRNAs)是一类小的非编码RNA，长度约为21-25个核苷酸。最近，一些研究人员证明，血浆miRNAs是多种癌症的敏感和特异的生物标志物。本研究的主要目的是探讨在GBM患者血浆中存在的miRNAs是否可以作为诊断生物标志物，并与胶质瘤的分类和临床治疗相关。采自50例患者和10例健康献血员的静脉血。采用实时荧光定量聚合酶链式反应检测血浆miRNAs水平。GBM患者血浆miR-21、miR-128和miR-342-3p水平较正常对照组明显升高，可作为胶质瘤与正常对照的鉴别诊断指标，具有较高的特异性和敏感性。然而，在脑膜瘤或垂体腺瘤等其他脑肿瘤患者中，这三种miRNAs没有明显变化。此外，在接受手术和放化疗的GBM患者中，这三种miRNAs的水平几乎恢复到正常水平。此外，miR-128和miR-342-3p的表达与胶质瘤的组织学分级呈正相关。这些发现表明，血浆特异性miRNAs有可能作为新的胶质瘤生物标志物使用，并可能在脑胶质瘤患者的临床治疗中有用。
Glioblastoma multiforme (GBM) is a highly malignant brain tumor with a poor prognosis. MicroRNAs (miRNAs) are a class of small non-coding RNAs, approximately 21–25 nucleotides in length. Recently, some researchers have demonstrated that plasma miRNAs are sensitive and specific biomarkers of various cancers. The primary aim of the study is to investigate whether miRNAs present in the plasma of GBM patients can be used as diagnostic biomarkers and are associated with glioma classification and clinical treatment. Plasma samples were attained by venipuncture from 50 patients and 10 healthy donors. Plasma levels of miRNAs were determined by real-time quantitative polymerase chain reaction. The plasma levels of miR-21, miR-128 and miR-342-3p were significantly altered in GBM patients compared to normal controls and could discriminate glioma from healthy controls with high specificity and sensitivity. However, these three miRNAs were not significantly changed in patients with other brain tumors such as meningioma or pituitary adenoma. Furthermore, the plasma levels of these three miRNAs in GBM patients treated by operation and chemo-radiation almost revived to normal levels. Finally, we also demonstrated that miR-128 and miR-342-3p were positively correlated with histopathological grades of glioma. These findings suggest that plasma specific miRNAs have potential use as novel biomarkers of glioma and may be useful in clinical management for glioma patients.
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影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
3.9
作者：
Cui, J. G.;Zhao, Y.;Lukiw, W. J.
通讯作者：
Lukiw, W. J.
DOI：
10.1083/jcb.200508014
发表时间：
2006-03-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Booth AM;Fang Y;Fallon JK;Yang JM;Hildreth JE;Gould SJ
通讯作者：
Gould SJ
影响因子：
21.3
作者：
Skog, Johan;Wuerdinger, Tom;van Rijn, Sjoerd;Meijer, Dimphna H.;Gainche, Laura;Sena-Esteves, Miguel;Curry, William T., Jr.;Carter, Bob S.;Krichevsky, Anna M.;Breakefield, Xandra O.
通讯作者：
Breakefield, Xandra O.
影响因子：
3.9
作者：
Conti, Alfredo;Aguennouz, M'Hammed;Tomasello, Francesco
通讯作者：
Tomasello, Francesco