Incubation temperature affects the development of calipash related traits and collagen deposition in embryos of soft-shelled turtle Pelodiscus sinensis
Incubation temperature affects the development of calipash related traits and collagen deposition in embryos of soft-shelled turtle Pelodiscus sinensis
复制标题
孵化温度影响中华鳖胚胎中甲鱼相关性状的发育和胶原沉积
DOI：
10.1016/j.aquaculture.2020.735546
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发表时间：
2020-11
期刊：
影响因子：
4.5
通讯作者：
钱国英
中科院分区：
文献类型：
--
作者：
李彩燕;杨捷;宋伟;任建东;方婵琳;钱国英
中华鳖（Pelodiscus sinensis）背甲周围有一层柔软而柔韧的围裙（calipash），其形成及其与细胞外基质的关系尚不清楚。在此，我们研究了在两种不同温度（26 °C和32 °C）下孵育的龟卵的龟卵质量性状和胶原蛋白沉积，以评估胶原蛋白在胚胎发生期间参与龟卵发育。结果表明，随着胚的发育，胚质量、胚长、胚重等与马蹄莲相关的性状总体上呈增加趋势。32 °C培养的胚胎在大多数发育阶段的胚胎体积、胼胝体重量和胶原蛋白含量均显著高于26 °C培养的胚胎。从发育阶段23开始观察到强烈的双折射颜色，并且在32 °C下孵育的胚胎中有更多的胶原纤维呈现偏振的橙子或红色。发现海龟calipash组织中I型胶原面积的百分比总体上增加，并且在32 °C下孵育的胚胎中具有显著更高的值。32 ℃孵育的第23期鳖卵中Ⅰ型胶原基因mRNA水平明显升高，说明在P.可诱导出sinensisembryos。本研究表明，在胚胎发育过程中，32 °C的孵育温度有利于胶原蛋白的沉积，并使其具有更好的形态特征。sinensis。这些数据提供了通过孵育温度变化来操纵哈里发性状发育的可能性。当试图在P中发育大的葫芦组织时，建议高的培养温度。sinensisaquacium。
Chinese soft-shelled turtlePelodiscus sinensispossesses a distinctive soft and pliable apron around its dorsal carapace termed calipash, of which the formation and its relation to extracellular matrix remain largely unclear. Herein, we investigated calipash quality traits and collagen deposition of turtle embryos from eggs incubated at two different temperatures (26 °C and 32 °C) to evaluate the involvement of collagen in calipash development during embryogenesis. We found that the calipash related traits including embryo mass, embryo length and calipash mass showed an overall increasing tendency as embryos developed. The positive correlation between collagen content and the above morphologic traits in calipash was also found. Embryos incubated at 32 °C incubation temperature had significant bigger embryo size, heavier calipash and higher collagen content at most developmental stages as compared with those embryos incubated at 26 °C. Strong birefringence color was observed from the starting of developmental stage 23 and there were much more collagen fibers presented polarized orange or red colors from embryos incubating at 32 °C. The percentage of type I collagen areas in turtle calipash tissue was found to increase overall and with significantly higher value in embryos incubated at 32 °C. The observed promoted mRNA level of type I collagen gene of turtle eggs incubated at 32 °C in developmental stage 23 indicated that the biosynthesis of collagen inP. sinensisembryos might be induced. The present work indicated that 32 °C incubation temperature is conducive to more collagen deposition and related more preferable calipash traits during the embryonic development ofP. sinensis. The data provided the possible manipulation of calipash trait development by incubation temperature changing. High incubation temperature is suggested when attempting to develop large calipash tissue inP. sinensisaquaculture.
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影响因子：
3.5
作者：
Ghislaine Morvan Dubois;Zofia Haftek;C. Crozet;R. Garrone;D. Le Guellec
通讯作者：
Ghislaine Morvan Dubois;Zofia Haftek;C. Crozet;R. Garrone;D. Le Guellec
DOI：
10.1007/bf00266843
发表时间：
1989-01-01
期刊：
HISTOCHEMISTRY
影响因子：
--
作者：
DAYAN, D;HISS, Y;WOLMAN, M
通讯作者：
WOLMAN, M
影响因子：
0.9
作者：
Tokita, M;Kuratani, S
通讯作者：
Kuratani, S
影响因子：
2.5
作者：
L. Alibardi
通讯作者：
L. Alibardi
影响因子：
3.8
作者：
Guillomot, M.;Campion, E.;Jammes, H.
通讯作者：
Jammes, H.