Suppression of Acute Ixodes scapularis-Induced Borrelia burgdorferi Infection using Tumor Necrosis Factor-α, Interleukin-2, and Interferon-γ
Suppression of Acute Ixodes scapularis-Induced Borrelia burgdorferi Infection using Tumor Necrosis Factor-α, Interleukin-2, and Interferon-γ
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使用肿瘤坏死因子-α、白细胞介素-2 和干扰素-γ 抑制肩胛硬蜱引起的急性伯氏疏螺旋体感染
DOI：
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
D. Fish
中科院分区：
文献类型：
--
作者：
N. Zeidner;M. Dreitz;D. Belasco;D. Fish
哺乳动物细胞因子的产生在蜱饲喂过程中已被证实下调。为了验证蜱喂养期间细胞因子的重建可以促进伯氏疏螺旋体的免疫遏制这一假设，进行了以下实验。 C3H/HeJ 小鼠在肩胛硬蜱附着后给予细胞因子 10 天。第 21 天，对耳部活检进行了伯氏疏螺旋体分析。聚合酶链反应分析表明，接受肿瘤坏死因子 (TNF)-α 治疗的小鼠的保护率为 95%。接受白细胞介素 (IL)-2 或干扰素 (IFN)-γ 治疗的小鼠的感染率为 30%-45%，而未经治疗的对照组小鼠的感染率为 83%。中和抗体、蛋白质印迹反应性和随后的保护之间没有发现相关性。在细胞因子条件培养基中培养伯氏疏螺旋体表明，TNF-α、IFN-γ 和 IL-2 对伯氏疏螺旋体没有细胞毒性。这些数据表明，细胞因子诱导的针对伯氏疏螺旋体感染的保护是免疫介导的，并且细胞免疫可能与针对肩胛伯氏菌诱导的感染的保护有关。
Down-regulation of mammalian cytokine production has been demonstrated during tick feeding. To examine the hypothesis that reconstitution of cytokines during tick feeding could facilitate immune containment of Borrelia burgdorferi, the following experiments were done. C3H/HeJ mice were given cytokines for 10 days after Ixodes scapularis attachment. At day 21, ear biopsies were analyzed for B. burgdorferi. Polymerase chain reaction analysis indicated a protection rate of 95% in mice receiving tumor necrosis factor (TNF)-alpha. Mice that received interleukin (IL)-2 or interferon (IFN)-gamma had infection rates of 30%-45% compared with 83% for untreated controls. No correlation was noted between neutralizing antibody, reactivity by Western blot, and subsequent protection. Culture of B. burgdorferi in cytokine-conditioned media indicated that TNF-alpha, IFN-gamma, and IL-2 were not cytotoxic for B. burgdorferi. These data suggest that cytokine-induced protection from B. burgdorferi infection was immune-mediated and that cellular immunity may be associated with protection from I. scapularis-induced infection.
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影响因子：
56.9
作者：
KARUPIAH, G;XIE, QW;MACMICKING, JD
通讯作者：
MACMICKING, JD
影响因子：
2.1
作者：
CROSS, ML;CUPP, MS;ENRIQUEZ, FJ
通讯作者：
ENRIQUEZ, FJ
莱姆病螺旋体从小鼠皮肤沉积部位的延迟传播。
DOI：
10.1093/infdis/166.4.827
发表时间：
1992
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Shih,CM;Pollack,RJ;Telford3rd,SR;Spielman,A
通讯作者：
Spielman,A
通过各种接种途径，Lewis 大鼠中伯氏疏螺旋体的相对感染性。
DOI：
10.4269/ajtmh.1991.44.135
发表时间：
1991
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
Moody,KD;Barthold,SW
通讯作者：
Barthold,SW
达米尼硬蜱：唾液抗补体活性。
影响因子：
2.1
作者：
Ribeiro,JM
通讯作者：
Ribeiro,JM