Regulation of gene expression in plants through miRNA inactivation.
Regulation of gene expression in plants through miRNA inactivation.
复制标题
DOI：
10.1371/journal.pone.0021330
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Heck GR
中科院分区：
文献类型：
--
作者：
Ivashuta S;Banks IR;Wiggins BE;Zhang Y;Ziegler TE;Roberts JK;Heck GR
真核生物具有复杂的RNA指导的基因表达调控网络，允许产生独特的基因表达模式。最近加入到基于RNA的基因调控库中的是miRNA靶诱饵，其是可以负调节miRNA活性的内源RNA。miRNA诱饵已被证明是了解植物和无脊椎动物中几个miRNA家族功能的有价值的工具。通过修饰miRNA活性对内源RNA调控网络进行工程化和精确操作也提供了实现增强植物发育或对环境胁迫的响应的期望结果的重要机会。在这里，我们报告的miRNA诱饵的表达作为单一或异聚体的不可切割的microRNA（miRNA）的网站嵌入在非蛋白编码或3′非翻译区的蛋白编码转录本可以调节一个或多个miRNA靶的表达。通过改变miRNA诱饵位点的序列，我们能够减弱miRNA的失活，这允许对天然miRNA靶标进行精细调节并产生理想范围的植物表型。因此，我们的研究结果表明，miRNA诱饵是一种灵活而强大的工具，不仅用于研究miRNA的功能，而且用于植物基因表达的靶向工程。对拟南芥转录组的计算分析揭示了许多潜在的miRNA诱饵，表明内源性诱饵可能在植物表达的自然调节中起重要作用。
Eukaryotic organisms possess a complex RNA-directed gene expression regulatory network allowing the production of unique gene expression patterns. A recent addition to the repertoire of RNA-based gene regulation is miRNA target decoys, endogenous RNA that can negatively regulate miRNA activity. miRNA decoys have been shown to be a valuable tool for understanding the function of several miRNA families in plants and invertebrates. Engineering and precise manipulation of an endogenous RNA regulatory network through modification of miRNA activity also affords a significant opportunity to achieve a desired outcome of enhanced plant development or response to environmental stresses. Here we report that expression of miRNA decoys as single or heteromeric non-cleavable microRNA (miRNA) sites embedded in either non-protein-coding or within the 3′ untranslated region of protein-coding transcripts can regulate the expression of one or more miRNA targets. By altering the sequence of the miRNA decoy sites, we were able to attenuate miRNA inactivation, which allowed for fine regulation of native miRNA targets and the production of a desirable range of plant phenotypes. Thus, our results demonstrate miRNA decoys are a flexible and robust tool, not only for studying miRNA function, but also for targeted engineering of gene expression in plants. Computational analysis of the Arabidopsis transcriptome revealed a number of potential miRNA decoys, suggesting that endogenous decoys may have an important role in natural modulation of expression in plants.
登录
查看更多内容
影响因子：
3.5
作者：
Sire, Christelle;Beatriz Moreno, Ana;San Segundo, Blanca
通讯作者：
San Segundo, Blanca
Mirbase：MicroRNA基因组学的工具。
影响因子：
14.9
作者：
Griffiths-Jones, Sam;Saini, Harpreet Kaur;van Dongen, Stijn;Enright, Anton J.
通讯作者：
Enright, Anton J.
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F
影响因子：
48
作者：
Ebert, Margaret S.;Neilson, Joel R.;Sharp, Phillip A.
通讯作者：
Sharp, Phillip A.