Microbial nitrification in throughfall of a Japanese cedar associated with archaea from the tree canopy.
Microbial nitrification in throughfall of a Japanese cedar associated with archaea from the tree canopy.
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DOI：
10.1186/s40064-016-3286-y
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Watanabe M
中科院分区：
文献类型：
--
作者：
Watanabe K;Kohzu A;Suda W;Yamamura S;Takamatsu T;Takenaka A;Koshikawa MK;Hayashi S;Watanabe M
为了探讨叶际微生物的硝化潜力，我们培养的穿透雨样品收集下的日本雪松（柳杉）的树冠，并分析了无机氮的转化样品。孵育4周后，未过滤穿透水中的硝酸盐浓度增加，但过滤样品中的硝酸盐浓度几乎保持不变（孔径：0.2和0.4 µm）。在未过滤的样品中，硝酸盐的δ18O和δ15N值在培养过程中降低。此外，古细菌氨单加氧酶亚基A（amoA）基因，参与氨的氧化，被发现在穿透样品，虽然betaproteobacteria amoA基因没有检测到。从C. japonica也来自古菌。相反，硝酸盐的生产，硝酸盐的同位素比下降，并存在amoA基因没有观察到从一个开放的区域收集的降雨样本。因此，微生物的硝化作用，发生在培养的穿透雨可能是由于氨氧化古菌被冲走的树冠降水。
To investigate the nitrification potential of phyllospheric microbes, we incubated throughfall samples collected under the canopies of Japanese cedar (Cryptomeria japonica) and analyzed the transformation of inorganic nitrogen in the samples. Nitrate concentration increased in the unfiltered throughfall after 4 weeks of incubation, but remained nearly constant in the filtered samples (pore size: 0.2 and 0.4 µm). In the unfiltered samples, δ18O and δ15N values of nitrate decreased during incubation. In addition, archaeal ammonia monooxygenase subunit A (amoA) genes, which participate in the oxidation of ammonia, were found in the throughfall samples, although betaproteobacterial amoA genes were not detected. The amoA genes recovered from the leaf surface of C. japonica were also from archaea. Conversely, nitrate production, decreased isotope ratios of nitrate, and the presence of amoA genes was not observed in rainfall samples collected from an open area. Thus, the microbial nitrification that occurred in the incubated throughfall is likely due to ammonia-oxidizing archaea that were washed off the tree canopy by precipitation.
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影响因子：
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