Non-microtubule tubulin-based backbone and subordinate components of postsynaptic density lattices.
Non-microtubule tubulin-based backbone and subordinate components of postsynaptic density lattices.
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DOI：
10.26508/lsa.202000945
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发表时间：
2021-07
影响因子：
4.4
通讯作者：
Tabuchi K
中科院分区：
文献类型：
--
作者：
Suzuki T;Terada N;Higashiyama S;Kametani K;Shirai Y;Honda M;Kai T;Li W;Tabuchi K
本研究提出了一个突触后密度（PSD）晶格模型，包括一个非微管蛋白为基础的骨干结构及其相关蛋白，包括各种PSD支架/衔接蛋白和其他PSD蛋白。开发了一种纯化方案来鉴定和分析突触后密度（PSD）晶格的组分蛋白，所述PSD晶格是中枢神经系统中兴奋性突触PSD的核心结构。通过突触质膜处理纯化“富集”和“精益”型PSD晶格，以通过综合鸟枪质谱法鉴定蛋白质组分，并将其分为最小必需细胞骨架（MEC）和非MEC组分。微管蛋白被认为是MEC的主要成分，与非微管微管蛋白广泛分布在纯化的PSD晶格。包埋后免疫金标记电镜观察证实PSD内及周围存在微管蛋白。非MEC蛋白包括各种典型的支架/接头PSD蛋白和其他类PSD蛋白。因此，本研究提供了一种新的PSD晶格模型，由非微管蛋白为基础的骨干和各种非MEC蛋白质。我们的研究结果表明，微管蛋白是一个关键组成部分，构建骨干和相关的组件是必不可少的多功能的PSD。
This study proposes a postsynaptic density (PSD) lattice model comprising a non-microtubule tubulin-based backbone structure and its associated proteins, including various PSD scaffold/adaptor proteins and other PSD proteins. A purification protocol was developed to identify and analyze the component proteins of a postsynaptic density (PSD) lattice, a core structure of the PSD of excitatory synapses in the central nervous system. “Enriched”- and “lean”-type PSD lattices were purified by synaptic plasma membrane treatment to identify the protein components by comprehensive shotgun mass spectrometry and group them into minimum essential cytoskeleton (MEC) and non-MEC components. Tubulin was found to be a major component of the MEC, with non-microtubule tubulin widely distributed on the purified PSD lattice. The presence of tubulin in and around PSDs was verified by post-embedding immunogold labeling EM of cerebral cortex. Non-MEC proteins included various typical scaffold/adaptor PSD proteins and other class PSD proteins. Thus, this study provides a new PSD lattice model consisting of non-microtubule tubulin-based backbone and various non-MEC proteins. Our findings suggest that tubulin is a key component constructing the backbone and that the associated components are essential for the versatile functions of the PSD.
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影响因子：
4.2
作者：
Cheng, Hui-Hsuan;Huang, Zu-Han;Chang, Yen-Chung
通讯作者：
Chang, Yen-Chung
影响因子：
16.2
作者：
Bosch M;Castro J;Saneyoshi T;Matsuno H;Sur M;Hayashi Y
通讯作者：
Hayashi Y
影响因子：
4.8
作者：
Carré, M;André, N;Braguer, D
通讯作者：
Braguer, D
影响因子：
2.5
作者：
Fiala, JC;Kirov, SA;Harris, KM
通讯作者：
Harris, KM
影响因子：
7
作者：
Ishihama, Y;Oda, Y;Mann, M
通讯作者：
Mann, M