Influence of linoleic acid-induced oxidative modifications on physicochemical changes and in vitro digestibility of porcine myofibrillar proteins
Influence of linoleic acid-induced oxidative modifications on physicochemical changes and in vitro digestibility of porcine myofibrillar proteins
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亚油酸诱导的氧化修饰对猪肌原纤维蛋白理化变化及体外消化率的影响
DOI：
10.1016/j.lwt.2014.12.037
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发表时间：
2015-05
期刊：
影响因子：
--
通讯作者：
Weizheng Sun
中科院分区：
文献类型：
--
作者：
Feibai Zhou;Mouming Zhao;Chun Cui;Weizheng Sun
Effects of linoleic acid-induced oxidation on the digestibility of myofibrillar proteins (MP) were investigated using anin vitrogastric-intestinal digestion model. The oxidative modifications of MP structure were evaluated by the contents of carbonyl and total sulfhydryls, surface hydrophobicity, SDS-PAGE and Fourier transform infrared spectroscopy (FTIR). Based on the results of digestion rate and digestibility, the MP digestion was strongly affected by the oxidation level, depending on the resultant protein structural changes. With the concentration of oxidized linoleic acid (OLA) below 3 mM, the digestion rate and digestibility of MP were markedly increased mainly due to enhanced protein flexibility and surface hydrophobicity. However, at high OLA concentrations (5–10 mM), the adverse effect in protein digestion was observed, which might be attributed to the accelerated protein aggregation. As for the digestion properties, oxidation lowered its overall nutritional value, as revealed by amino acid composition analysis. Nevertheless, digestion product of MP prior treated with OLA below 1 mM exhibited an enhanced antioxidant activity with a higher oxygen radical absorbance capacity (ORAC) value. This improvement might be mainly attributed to the partial degradation of myosin and troponin-T, which favored their recognition during the intestinal tract, thus increasing the content of Cys and Tyr.
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DOI：
10.4324/9780429036279-4
发表时间：
1986-08
期刊：
WHO chronicle
影响因子：
--
作者：
S. Premakumari
通讯作者：
S. Premakumari
DOI：
10.1016/s0021-9258(18)45598-6
发表时间：
1987-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
C. Oliver;B. Ahn;E. Moermans;S. Goldstein;E. Stadtman
通讯作者：
C. Oliver;B. Ahn;E. Moermans;S. Goldstein;E. Stadtman
影响因子：
6.1
作者：
Sante-Lhoijtellier, Veronique;Astrijc, Thierry;Gatellier, Philippe
通讯作者：
Gatellier, Philippe
影响因子：
6.1
作者：
Zhu, Lijuan;Chen, Jie;Xiong, Youling L.
通讯作者：
Xiong, Youling L.
影响因子：
7.1
作者：
Feibai Zhou;Mouming Zhao;Haifeng Zhao;Weizheng Sun;Chun Cui
通讯作者：
Feibai Zhou;Mouming Zhao;Haifeng Zhao;Weizheng Sun;Chun Cui