IL-1β and TNFα promote monocyte viability through the induction of GM-CSF expression by rheumatoid arthritis synovial fibroblasts.
IL-1β and TNFα promote monocyte viability through the induction of GM-CSF expression by rheumatoid arthritis synovial fibroblasts.
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DOI：
10.1155/2014/241840
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发表时间：
2014
影响因子：
4.6
通讯作者：
Le Goff B
中科院分区：
文献类型：
--
作者：
Darrieutort-Laffite C;Boutet MA;Chatelais M;Brion R;Blanchard F;Heymann D;Le Goff B
背景。巨噬细胞和滑膜成纤维细胞（SF）是参与类风湿关节炎（RA）发病的两个主要细胞。SF可能是驱动巨噬细胞存活和活化的细胞因子和生长因子的来源。我们研究了SF对单核细胞活力和表型的影响。方法。从RA患者的滑膜组织中分离SF，从健康供者的外周血中分离CD14+细胞。SF条件培养基在TNFα或IL-1β刺激或不刺激培养24小时后收集。流式细胞术观察巨噬细胞极化。结果。与单独培养的CD14+细胞相比，SF条件培养基显著提高了60%的单核细胞活力（P < 0.001）。IL-1β和tnf - α刺激SF的条件培养基增强了这种作用。将GM-CSF添加到IL-1β和tnf - α刺激的SF的条件培养基中，可显著降低30%的单核细胞活力（P < 0.001），而M-CSF和il - 34阻断抗体则没有作用。最后，在SF条件培养基中培养的单核细胞没有表现出特定的M1或M2表型。结论。总的来说，促炎细胞因子（IL-1β和TNFα）刺激的类风湿性关节炎滑膜成纤维细胞通过GM-CSF促进单核细胞活力，但不诱导特异性巨噬细胞极化。
Background. Macrophages and synovial fibroblasts (SF) are two major cells implicated in the pathogenesis of rheumatoid arthritis (RA). SF could be a source of cytokines and growth factors driving macrophages survival and activation. Here, we studied the effect of SF on monocyte viability and phenotype. Methods. SF were isolated from synovial tissue of RA patients and CD14+ cells were isolated from peripheral blood of healthy donors. SF conditioned media were collected after 24 hours of culture with or without stimulation with TNFα or IL-1β. Macrophages polarisation was studied by flow cytometry. Results. Conditioned medium from SF significantly increased monocytes viability by 60% compared to CD14+ cells cultured in medium alone (P < 0.001). This effect was enhanced using conditioned media from IL-1β and TNFα stimulated SF. GM-CSF but not M-CSF nor IL34 blocking antibodies was able to significantly decrease monocyte viability by 30% when added to the conditioned media from IL-1β and TNFα stimulated SF (P < 0.001). Finally, monocyte cultured in presence of SF conditioned media did not exhibit a specific M1 or M2 phenotype. Conclusion. Overall, rheumatoid arthritis synovial fibroblasts stimulated with proinflammatory cytokines (IL-1β and TNFα) promote monocyte viability via GM-CSF but do not induce a specific macrophage polarization.
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在类风湿滑膜滑膜成纤维细胞激活IL-17和TNFALPHA后，长时间的粒细胞巨噬细胞刺激性刺激因子依赖性，中性粒细胞存活。
影响因子：
4.9
作者：
Parsonage, Greg;Filer, Andrew;Bik, Magdalena;Hardie, Debbie;Lax, Sian;Howlett, Katherine;Church, Leigh D.;Raza, Karim;Wong, See-Heng;Trebilcock, Emily;Scheel-Toellner, Dagmar;Salmon, Mike;Lord, Janet M.;Buckley, Christopher D.
通讯作者：
Buckley, Christopher D.
影响因子：
5
作者：
Li, Jun;Hsu, Hui-Chen;Mountz, John D.
通讯作者：
Mountz, John D.
影响因子：
12.8
作者：
Dickerson TJ;Suzuki E;Stanecki C;Shin HS;Qui H;Adamopoulos IE
通讯作者：
Adamopoulos IE
影响因子：
4.9
作者：
Ambarus, Carmen A.;Noordenbos, Troy;Baeten, Dominique L. P.
通讯作者：
Baeten, Dominique L. P.
趋化因子在类风湿关节炎中白细胞 - 基质相互作用中的作用。
DOI：
10.2741/2874
发表时间：
2008-01-01
期刊：
Frontiers in bioscience : a journal and virtual library
影响因子：
--
作者：
Filer A;Raza K;Salmon M;Buckley CD
通讯作者：
Buckley CD