The complete mitochondrial genome of the bag-shelter moth Ochrogaster lunifer (Lepidoptera, Notodontidae).
The complete mitochondrial genome of the bag-shelter moth Ochrogaster lunifer (Lepidoptera, Notodontidae).
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DOI：
10.1186/1471-2164-9-331
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发表时间：
2008-07-15
期刊：
影响因子：
4.4
通讯作者：
Negrisolo E
中科院分区：
文献类型：
--
作者：
Salvato P;Simonato M;Battisti A;Negrisolo E
动物线粒体基因组的知识对于理解它们的分子进化以及系统发育和种群遗传学研究都是非常重要的。鳞翅目包括超过16万种描述的物种，是最大的昆虫目之一。到目前为止，只有9个鳞翅目昆虫的线粒体DNA被完全测序，另外两个已经部分测序。此外，对分类单元的采样也很少。因此，鳞翅目有丝分裂组学的发展迫切需要从广泛的分类单元取样中获得新的基因组。在目前的工作中，我们描述了月亮蛾的线粒体基因组。罗非鱼线粒体基因组为环状分子，全长15593个碱基。它包括通常存在于动物线粒体基因组中的全部37个基因。它还含有7个基因间隔区。新测序的基因组的基因序列与鳞翅目的典型序列相同，与昆虫祖先类型的trnM的位置不同。其α链A+T含量为77.84%，是已知鳞翅目昆虫基因组中含量最低的。在现有的昆虫翼目基因组中，新月牛的线粒体基因组表现出最明显的C-偏斜。蛋白质编码基因具有典型的线粒体起始密码子，除了cox1，它呈现一种不寻常的CGA。在鳞翅目昆虫中，新月虫基因组表现出较少偏向的同义密码子使用。比较基因组学分析确定atp6、cox1、cox2为cox3，cob、nad1、nad2、nad4和nad5为群体遗传学/系统发育研究的潜在标记。新月牛线粒体基因组的一个特殊特征是，基因间隔区大多由重复序列组成。月斑夜蛾的线粒体基因组是夜蛾总科的第一个代表，约占所描述鳞翅目总数的40%。新的基因组与其他已知的鳞翅目基因组有许多共同的特征。然而，它的不同之处在于其A+T含量较低，并带有明显的C偏。与其他鳞翅目昆虫基因组相比，它在同义密码子使用上的偏见较小。对鳞翅目昆虫线粒体基因组的比较进化分析使以前被忽视的编码基因成为潜在的系统发育标记。新月稻基因组基因间隔区中重复元件的存在支持DNA滑移作为复制过程中产生间隔区的可能机制。
Knowledge of animal mitochondrial genomes is very important to understand their molecular evolution as well as for phylogenetic and population genetic studies. The Lepidoptera encompasses more than 160,000 described species and is one of the largest insect orders. To date only nine lepidopteran mitochondrial DNAs have been fully and two others partly sequenced. Furthermore the taxon sampling is very scant. Thus advance of lepidopteran mitogenomics deeply requires new genomes derived from a broad taxon sampling. In present work we describe the mitochondrial genome of the moth Ochrogaster lunifer. The mitochondrial genome of O. lunifer is a circular molecule 15593 bp long. It includes the entire set of 37 genes usually present in animal mitochondrial genomes. It contains also 7 intergenic spacers. The gene order of the newly sequenced genome is that typical for Lepidoptera and differs from the insect ancestral type for the placement of trnM. The 77.84% A+T content of its α strand is the lowest among known lepidopteran genomes. The mitochondrial genome of O. lunifer exhibits one of the most marked C-skew among available insect Pterygota genomes. The protein-coding genes have typical mitochondrial start codons except for cox1 that present an unusual CGA. The O. lunifer genome exhibits the less biased synonymous codon usage among lepidopterans. Comparative genomics analysis study identified atp6, cox1, cox2 as cox3, cob, nad1, nad2, nad4, and nad5 as potential markers for population genetics/phylogenetics studies. A peculiar feature of O. lunifer mitochondrial genome it that the intergenic spacers are mostly made by repetitive sequences. The mitochondrial genome of O. lunifer is the first representative of superfamily Noctuoidea that account for about 40% of all described Lepidoptera. New genome shares many features with other known lepidopteran genomes. It differs however for its low A+T content and marked C-skew. Compared to other lepidopteran genomes it is less biased in synonymous codon usage. Comparative evolutionary analysis of lepidopteran mitochondrial genomes allowed the identification of previously neglected coding genes as potential phylogenetic markers. Presence of repetitive elements in intergenic spacers of O. lunifer genome supports the role of DNA slippage as possible mechanism to produce spacers during replication.
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