Candidate olfaction genes identified within the Helicoverpa armigera Antennal Transcriptome.

Candidate olfaction genes identified within the Helicoverpa armigera Antennal Transcriptome.
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棉铃虫触角转录组中鉴定出的候选嗅觉基因

DOI:
10.1371/journal.pone.0048260
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发表时间:
2012
期刊:
影响因子:
3.7
通讯作者:
Wang G
Wang G
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Liu Y;Gu S;Zhang Y;Guo Y;Wang G

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昆虫嗅觉对昆虫的生存极其重要,介导诸如寄主偏好、配偶选择和产卵地点选择等关键行为。嗅觉信号转导通路中涉及多种触角蛋白。其中,气味受体(OR)和离子型受体(IR)赋予嗅觉感觉神经元反应的特异性。在这项研究中,我们确定了嗅觉基因库的经济上重要的农业害虫蛾,棉铃虫,成年男性和女性的触角转录组组装。在男性和女性的触角转录组,我们确定了总共47个或候选基因包含6个信息素受体候选人。此外,12 IR基因以及26气味结合蛋白和12化学传感蛋白进行了注释。我们的结果允许对大部分传统ORs谱系和新报告的IR进行系统的功能分析,这些IR介导H的关键嗅觉介导行为。armigera。
Antennal olfaction is extremely important for insect survival, mediating key behaviors such as host preference, mate choice, and oviposition site selection. Multiple antennal proteins are involved in olfactory signal transduction pathways. Of these, odorant receptors (ORs) and ionotropic receptors (IRs) confer specificity on olfactory sensory neuron responses. In this study, we identified the olfactory gene repertoire of the economically important agricultural pest moth, Helicoverpa armigera, by assembling the adult male and female antennal transcriptomes. Within the male and female antennal transcriptomes we identified a total of 47 OR candidate genes containing 6 pheromone receptor candidates. Additionally, 12 IR genes as well as 26 odorant-binding proteins and 12 chemosensory proteins were annotated. Our results allow a systematic functional analysis across much of conventional ORs repertoire and newly reported IRs mediating the key olfaction-mediated behaviors of H. armigera.
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