Haplotype diversity of VvTFL1A gene and association with cluster traits in grapevine (V. vinifera).
Haplotype diversity of VvTFL1A gene and association with cluster traits in grapevine (V. vinifera).
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DOI：
10.1186/s12870-014-0209-3
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发表时间：
2014-08-05
影响因子：
5.3
通讯作者：
Martinez-Zapater JM
中科院分区：
文献类型：
--
作者：
Fernandez L;Le Cunff L;Tello J;Lacombe T;Boursiquot JM;Fournier-Level A;Bravo G;Lalet S;Torregrosa L;This P;Martinez-Zapater JM
拟南芥中，TFL 1和LFY的相互作用似乎决定了花序的结构。在一个平行的方式，VvTFL 1A，葡萄藤TFL 1同源物的过表达，导致延迟开花和生产的一个分枝簇在重复生殖分生组织（RRM）体细胞变种的栽培品种Carignan。分析该基因对栽培葡萄藤（葡萄属vinifera L.）亚种vinifera）的核苷酸多样性进行了表征，并对检测到的序列多态性与物候和聚类性状进行了关联分析。总共3.6 kb的VvTFL 1A基因（包括其启动子）在140个个体的核心集合中进行测序，该核心集合旨在最大化农艺相关性状的表型变异。VvTFL 1A在此集合内的核苷酸变异在启动子和内含子序列中比在外显子区域中更高;在外显子区域中，很少有多态性与编码序列的高度保守性一致。VvTFL 1A单倍型网络的表征确定了三个主要的单倍型群，与地理起源和使用的品种，可以对应于三个主要的祖先等位基因或进化分支，基于连锁不平衡的突变的存在。聚类性状的遗传关联研究揭示了INDEL多态性的存在，分别解释了开花时间、聚类宽度和浆果重量的16%、13%和25%，并构建了三个单倍型群。至少有三个主要的VvTFL 1A单倍型群存在于栽培葡萄，这是由三个主要的多态性LD块的存在下定义的，并与开花时间，簇宽度和浆果大小的特征表型值。单倍型群之间的表型差异与东部和西部葡萄藤品种之间观察到的差异是一致的，可能是由于在驯化过程中使用不同的遗传库，以及不同的选择压力表和葡萄酒品种的发展，分别。总而言之，这些结果是一致的葡萄葡萄藤表型多样性的分类，主要是基于集群和浆果形态，以及最近的结果在栽培葡萄藤的遗传多样性结构。
Interaction between TERMINAL FLOWER 1 (TFL1) and LEAFY (LFY) seem to determine the inflorescence architecture in Arabidopsis. In a parallel way, overexpression of VvTFL1A, a grapevine TFL1 homolog, causes delayed flowering and production of a ramose cluster in the reiterated reproductive meristem (RRM) somatic variant of cultivar Carignan. To analyze the possible contribution of this gene to cluster phenotypic variation in a diversity panel of cultivated grapevine (Vitis vinifera L. subsp. vinifera) its nucleotide diversity was characterized and association analyses among detected sequence polymorphisms and phenology and cluster traits was carried out. A total of 3.6 kb of the VvTFL1A gene, including its promoter, was sequenced in a core collection of 140 individuals designed to maximize phenotypic variation at agronomical relevant traits. Nucleotide variation for VvTFL1A within this collection was higher in the promoter and intron sequences than in the exon regions; where few polymorphisms were located in agreement with a high conservation of coding sequence. Characterization of the VvTFL1A haplotype network identified three major haplogroups, consistent with the geographic origins and the use of the cultivars that could correspond to three major ancestral alleles or evolutionary branches, based on the existence of mutations in linkage disequilibrium. Genetic association studies with cluster traits revealed the presence of major INDEL polymorphisms, explaining 16%, 13% and 25% of flowering time, cluster width and berry weight, respectively, and also structuring the three haplogroups. At least three major VvTFL1A haplogroups are present in cultivated grapevines, which are defined by the presence of three main polymorphism LD blocks and associated to characteristic phenotypic values for flowering time, cluster width and berry size. Phenotypic differences between haplogroups are consistent with differences observed between Eastern and Western grapevine cultivars and could result from the use of different genetic pools in the domestication process as well as different selection pressures on the development of table and wine cultivars, respectively. Altogether, these results are coherent with previous classifications of grapevine phenotypic diversity mainly based on cluster and berry morphotypes as well as with recent results on the structure of genetic diversity in cultivated grapevine.
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影响因子：
11.6
作者：
Hanano, Shigeru;Goto, Koji
通讯作者：
Goto, Koji
影响因子：
5.1
作者：
Jose Carmona, Maria;Calonje, Myriam;Miguel Martinez-Zapater, Jose
通讯作者：
Miguel Martinez-Zapater, Jose
影响因子：
7.2
作者：
Fernandez, Lucie;Torregrosa, Laurent;Martinez-Zapater, Jose M.
通讯作者：
Martinez-Zapater, Jose M.
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
56.9
作者：
COLLINS, FS;DRUMM, ML;IANNUZZI, MC
通讯作者：
IANNUZZI, MC