Nerve growth factor enhances neurite arborization of adult sensory neurons; a study i single-cell culture
Nerve growth factor enhances neurite arborization of adult sensory neurons; a study i single-cell culture
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神经生长因子增强成年感觉神经元的轴突树枝化；
DOI：
10.1016/0006-8993(90)90491-s
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Akira Takahashi
中科院分区：
文献类型：
--
作者：
T. Yasuda;G. Sobue;Takayuki Ito;T. Mitsuma;Akira Takahashi
神经生长因子（NGF）是一种公认的交感神经和感觉神经元发育过程中的营养因子。然而，很少有人知道，是所需的成熟的外周神经元的分化表型的维持或调节。由于目前已知营养因子（包括NGF）由非神经元细胞（如许旺细胞和成纤维细胞）分泌，因此需要高度纯的神经元培养物来评估营养因子对神经元的直接作用。我们已经开发了一个单一的神经元培养新生和成年大鼠背根神经节在无血清条件下，并估计神经生长因子的感觉神经元的形态几何学的主要影响。我们发现，神经生长因子促进新生感觉神经元的轴突长度，而不是促进树枝化（分支的神经突），而在成人成熟的神经元中，神经生长因子显着增强神经突树枝化，而不是最大的神经突延伸，距离从细胞索马的最大边缘的领土的神经突延伸。神经生长因子能显著增加新生和成年神经元的单位长度神经突总数。神经生长因子还增加了神经元索马的大小独立于神经元的成熟。新生感觉神经元倾向于死亡，在1周内，尽管存在的NGF。相反，一些成年感觉神经元在没有NGF的情况下存活超过2周。这些结果表明，神经生长因子比简单的加速预先存在的发展计划在成熟阶段，并在成人感觉神经元中的神经突起树枝化的促进表明，神经生长因子可能有一些周围神经再生通过促进轴突发芽的作用。
Nerve growth factor (NGF) is a well-established trophic factor of sympathetic and sensory neurons during development. NGF is, however, little known to be required for the maintenance or regulation of differentiated phenotypes of matured peripheral neurons. Since trophic factors, including NGF, are currently known to be secreted by non-neuronal cells, like Schwann cells and fibroblasts, a highly pure-neuron culture is required to assess the direct action of trophic factors on neurons. We have developed a single-neuron culture from neonatal and adult rat dorsal root ganglia in serum-free conditions, and estimated the primary effect of NGF on the morphological geometry of sensory neurons. We found that NGF promoted the neurite length of neonatal sensory neurons, rather than promoting arborization (branching of neurites), while in adult matured neurons NGF significantly enhanced neurite arborizations, rather than the maximal neurite extension, distance from the cell soma to the maximum margin of the territory of neurite extension. Total neurite per length, the summed length of all neurites per neuron was significantly increased by NGF in both neonatal and adult neurons. NGF also increased the size of neuronal soma independent of neuronal maturation. Neonatal sensory neurons tended to die in 1 week despite the presence of NGF. In contrast, some adult sensory neurons were alive for more than 2 weeks in the absence of NGF. These results indicate that NGF more than simple accelerates a pre-existing development program in the matured stage, and that the promotion of neurite arborization by NGF in adult sensory neurons suggests that NGF may have some role in peripheral nerve regeneration via promotion of axonal sprouting.
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神经生长因子与层粘连蛋白或纤连蛋白之间的合作促进感觉神经元存活和神经突生长。
影响因子：
2.9
作者：
Millaruelo,AI;Nieto-Sampedro,M;Cotman,CW
通讯作者：
Cotman,CW
影响因子：
2.7
作者：
ROGERS, SL;LETOURNEAU, PC;FURCHT, LT
通讯作者：
FURCHT, LT
单克隆抗体可识别单个神经丝三联蛋白。
DOI：
10.1073/pnas.79.19.6089
发表时间：
1982
影响因子：
11.1
作者：
Lee,V;Wu,HL;Schlaepfer,WW
通讯作者：
Schlaepfer,WW
体外睫状神经节神经元的神经突生长参数：层粘连蛋白、神经鞘瘤多聚鸟氨酸结合神经突促进因子和睫状神经营养因子的影响。
影响因子：
2.9
作者：
Davis,GE;Manthorpe,M;Varon,S
通讯作者：
Varon,S
DOI：
10.1073/pnas.84.21.7768
发表时间：
1987-11
影响因子：
11.1
作者：
S. Porter;L. Glaser;R. Bunge
通讯作者：
S. Porter;L. Glaser;R. Bunge