Enhanced Optical Collection of Micro- and Nanovesicles in the Presence of Gold Nanoparticles.
Enhanced Optical Collection of Micro- and Nanovesicles in the Presence of Gold Nanoparticles.
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DOI：
10.1021/acsomega.8b00033
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发表时间：
2018-03-31
期刊：
影响因子：
4.1
通讯作者：
Kaneta T
中科院分区：
文献类型：
--
作者：
Kuboi M;Takeyasu N;Kaneta T
我们描述了一种使用激光束的光学压力在玻璃基底上收集微囊泡和纳米囊泡的方法。激光束聚焦在玻璃基板上，玻璃基板夹着含有使用磷脂制备的囊泡的溶液。在囊泡表面产生的光学压力将它们拉到光束的中心，在那里它们在玻璃表面上形成聚集体。用缓冲溶液制备的囊泡通过吸附到玻璃表面上成功地收集，而用纯水制备的囊泡没有表现出这样的趋势。收集一定量囊泡所需的时间与其浓度成反比。为了提高收集效率，我们将金纳米颗粒添加到囊泡溶液中。向溶液中加入金纳米颗粒使收集时间减少到没有它的十分之一，这是由于溶液中金纳米颗粒吸收产生的热促进了热混合，以及金纳米颗粒诱导的光散射增强。与金纳米粒子耦合的囊泡的光学收集显示了在生物流体中收集痕量细胞外囊泡的希望。
We describe a process for collecting micro- and nanovesicles on a glass substrate using the optical pressure of a laser beam. The laser beam was focused on a glass substrate that sandwiched a solution containing vesicles prepared using a phospholipid. The optical pressure generated at the surface of the vesicles pulled them into the center of the beam where they formed an aggregate on the glass surface. The vesicles prepared with a buffer solution were successfully collected via adsorption onto the glass surface, whereas the vesicles prepared with pure water exhibited no such tendency. The time required to collect a certain amount of vesicles was inversely proportional to their concentration. To enhance the collection efficiency, we added gold nanoparticles to the vesicle solution. The addition of gold nanoparticles into the solution reduced the collection time to one-tenth of that without it, and this was attributed to thermal mixing promoted by the heat generated by the absorption from the gold nanoparticles in the solution, as well as to an enhancement of light scattering induced by the gold nanoparticles. The optical collection of vesicles coupled with gold nanoparticles shows a promise for the collection of trace amounts of extracellular vesicles in biological fluids.
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