Molecular epidemiology and seroprevalence in asymptomatic Plasmodium falciparum infections of Malagasy pregnant women in the highlands.
Molecular epidemiology and seroprevalence in asymptomatic Plasmodium falciparum infections of Malagasy pregnant women in the highlands.
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DOI：
10.1186/s12936-015-0704-5
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发表时间：
2015-05-03
期刊：
影响因子：
3
通讯作者：
Schwarz NG
中科院分区：
文献类型：
--
作者：
Maïga-Ascofaré O;Rakotozandrindrainy R;Girmann M;Hahn A;Randriamampionona N;Poppert S;May J;Schwarz NG
马达加斯加的疟疾流行病学分为四个不同的地区，从高地的不稳定季节性传播到沿海的高流行性常年传播地区。马达加斯加的大多数疟疾研究都集中在有症状的儿童身上。然而，由于在一些半免疫水平相应较低的地区传播率较低，成年人也面临风险，尤其是孕妇。这项研究的目的是获得关于孕妇疟疾感染遗传流行病学的信息，以便为马达加斯加的疟疾控制和消除方案提供信息。2010年5月至8月，我们在六个地点进行了针对健康孕妇的横断面调查，其中三个地点在沿海地区，三个地点在海拔850- 1 300米的高地。1244份血样通过免疫荧光试验筛查抗恶性疟原虫抗体，并通过实时PCR筛查疟疾感染。氯喹和磺胺嘧啶-乙胺嘧啶抗性标记的流行率也确定在所有恶性疟原虫样本的PCR-RFLP以及多重感染通过基因分型6个中性微卫星。在高原地区，67.4%的妇女有抗恶性疟原虫抗体，9.2%的妇女携带寄生虫，在沿海地区，这两个比例分别为95.6%和14.8%。在高原和沿海地区，感染孕妇平均检出1.2个克隆，1.5个克隆。高原地区单克隆感染率（85.4%）高于沿海地区（61.8%）。磺胺嘧啶-乙胺嘧啶的耐药标记只存在于两个网站。马达加斯加高地人群感染疟疾寄生虫后会产生免疫力，但这些人群也可能成为流行病的储存库。本文的在线版本（doi：10.1186/s12936-015-0704-5）包含补充材料，可供授权用户使用。
Malaria epidemiology in Madagascar is classified into four different areas, ranging from unstable seasonal transmission in the highlands to hyperendemic perennial transmission areas in the costal level. Most malaria studies in Madagascar are focused on symptomatic children. However, because of the low transmission in some areas with correspondingly low level of semi-immunity, adults are also at risk, in particular pregnant women. The objective of this study was to gain information on the genetic epidemiology of malarial infections in pregnant women in order to provide information for malaria control and elimination programmes in Madagascar. Between May and August 2010, we carried out cross-sectional surveys targeting healthy pregnant women in six locations, three in the coastal area and three in the highlands at 850–1300 m. 1244 blood samples were screened for anti-Plasmodium falciparum antibodies by immunofluorescence test and for malarial infection by realtime-PCR. The prevalence of chloroquine and sulphadoxine-pyrimethamine resistance markers was also determined in all Plasmodium falciparum samples by PCR-RFLP as well as the multiplicity of infection through genotyping six neutral microsatellites. In the highlands, 67.4% of the women presented antibodies against Plasmodium falciparum and 9.2% were carrying parasites, at the coast 95.6% and 14.8%, respectively. In the mean, 1.2 clones were detected in infected pregnant woman in the highlands and 1.5 at the coast. A higher level of monoclonal infections was found in the highlands (85.4%) compared to the coast (61.8%). Resistance markers for sulphadoxine-pyrimethamine were present only in two sites. Immunity is triggered in Malagasy highland populations when they are infected with malaria parasites, but these populations could also serve as a reservoir for epidemics. The online version of this article (doi:10.1186/s12936-015-0704-5) contains supplementary material, which is available to authorized users.
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影响因子：
11.8
作者：
Schwarz NG;Girmann M;Randriamampionona N;Bialonski A;Maus D;Krefis AC;Njarasoa C;Rajanalison JF;Ramandrisoa HD;Randriarison ML;May J;Schmidt-Chanasit J;Rakotozandrindrainy R
通讯作者：
Rakotozandrindrainy R
影响因子：
3.7
作者：
Andriantsoanirina V;Ratsimbasoa A;Bouchier C;Tichit M;Jahevitra M;Rabearimanana S;Raherinjafy R;Mercereau-Puijalon O;Durand R;Ménard D
通讯作者：
Ménard D
影响因子：
3.7
作者：
Ouedraogo, Andre Lin;Bousema, Teun;Sauerwein, Robert
通讯作者：
Sauerwein, Robert
影响因子：
4.9
作者：
Andriantsoanirina, Valerie;Ratsimbasoa, Arsene;Menard, Didier
通讯作者：
Menard, Didier
影响因子：
4.9
作者：
Dahlstroem, Sabina;Aubouy, Agnes;Houze, Sandrine
通讯作者：
Houze, Sandrine