Comparison of cellular toxicity caused by ambient ultrafine particles and engineered metal oxide nanoparticles.
Comparison of cellular toxicity caused by ambient ultrafine particles and engineered metal oxide nanoparticles.
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环境超细颗粒和工程金属氧化物纳米颗粒引起的细胞毒性的比较
DOI：
10.1186/s12989-015-0082-8
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发表时间：
2015-03-19
影响因子：
10
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
Lu S;Zhang W;Zhang R;Liu P;Wang Q;Shang Y;Wu M;Donaldson K;Wang Q
纳米技术的发展引发了人们对暴露于纳米颗粒（NP）和超细颗粒（UFP）对健康影响的担忧。 NP 和 UFP 的毒理学数据可以为支持制定降低颗粒暴露风险的法规提供证据。我们试图提供基础数据来确定环境 UFP 和工程金属氧化物纳米粒子（ZnO、NiO 和 CeO2）诱导的细胞毒性差异。使用纳米微孔均匀沉积物冲击器对 UFP 进行采样。通过扫描电子显微镜和透射电子显微镜研究了 UFP 和纳米金属氧化物颗粒的物理化学表征。通过综合方法评估不同颗粒诱导的细胞毒性，并在 A549 细胞暴露于颗粒后进行比较。所有测量到的颗粒在浓度范围为 25 至 200 μg/mL 时都可能损伤 A549 细胞。最低的存活率和最高的乳酸脱氢酶水平是由纳米 ZnO 颗粒引起的，但在用 200 μg/mL 环境细颗粒物 (PM1.8) 可溶性部分处理的细胞中观察到最高水平的细胞内活性氧 (ROS) 和细胞凋亡百分比。与环境粗颗粒和 UFP 相比，相对较高浓度的人为金属（包括 Zn、Ni、Fe 和 Cu）可能是细小环境颗粒毒性较高的原因。发现选定的重金属（Zn、Ni、Fe 和 Cu）位于 A549 细胞的核周和细胞质区域。周围颗粒中金属的分布模式表明，A549细胞中金属的分布不均匀，遵循Cu>Zn>Fe>Ni的模式，表明Cu比其他金属更容易被A549细胞吸收。低浓度的金属纳米颗粒氧化物和UFP可能会损害细胞，但与环境颗粒相比，制造的金属氧化物纳米颗粒对肺细胞的毒性并不高。 A549 细胞中重金属的局部浓度效应以及颗粒诱导的氧化应激可能是造成细胞损伤的原因。本文的在线版本 (doi:10.1186/s12989-015-0082-8) 包含补充材料，可供授权用户使用。
The development of nanotechnology has spurred concerns about the health effects of exposure to nanoparticles (NPs) and ultrafine particles (UFPs). Toxicological data on NPs and UFPs may provide evidence to support the development of regulations to reduce the risk of particle exposure. We tried to provide fundamental data to determine differences in cytotoxicity induced by ambient UFPs and engineered metal oxide NPs (ZnO, NiO, and CeO2). UFPs were sampled by using of a nano micro-orifice uniform deposit impactor. Physicochemical characterization of the UFPs and nano metal oxide particles were studied by scanning electron microscopy and transmission electron microscopy. Cellular toxicity induced by the different particles was assessed by using of comprehensive approaches and compared after A549 cells were exposured to the particles. All of the measured particles could damage A549 cells at concentrations ranging from 25 to 200 μg/mL. The lowest survival ratio and the highest lactate dehydrogenase level were caused by nano-ZnO particles, but the highest levels of intracellular reactive oxygen species (ROS) and percentages of apoptosis were observed in cells treated with the soluble fraction of ambient fine particles (PM1.8) at 200 μg/mL. Relatively high concentrations of anthropogenic metals, including Zn, Ni, Fe, and Cu, may be responsible for the higher toxicity of fine ambient particles compared with the ambient coarse particles and UFPs. The selected heavy metals (Zn, Ni, Fe, and Cu) were found to be located in the perinuclear and cytoplasmic areas of A549 cells. The distribution pattern of metals from ambient particles showed that distributions of the metals in A549 cells were not uniform and followed the pattern Cu > Zn > Fe > Ni, suggesting that Cu was absorbed by A549 cells more easily than the other metals. Metal nanoparticles oxides and UFPs at low concentration could damage to cells, but the manufactured metal oxide nanoparticles are not highly toxic to lung cells compared to environmental particles. The local concentration effect of heavy metals in A549 cells, as well as the induction of oxidative stress by the particles, may be responsible for the damage observed to the cells. The online version of this article (doi:10.1186/s12989-015-0082-8) contains supplementary material, which is available to authorized users.
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影响因子：
11.2
作者：
Trouiller B;Reliene R;Westbrook A;Solaimani P;Schiestl RH
通讯作者：
Schiestl RH
影响因子：
17.1
作者：
Xia, Tian;Kovochich, Michael;Liong, Monty;Meng, Huan;Kabehie, Sanaz;George, Saji;Zink, Jeffrey I.;Nel, Andre E.
通讯作者：
Nel, Andre E.
影响因子：
--
作者：
Carter, Elizabeth A.;Rayner, Benjamin S.;Lay, Peter A.
通讯作者：
Lay, Peter A.
纳米氧化锌颗粒诱导神经干细胞凋亡
影响因子：
3.5
作者：
Deng, Xiaoyong;Luan, Qixia;Jiao, Zheng
通讯作者：
Jiao, Zheng
影响因子：
4.5
作者：
Donaldson, K;Li, XY;MacNee, W
通讯作者：
MacNee, W