GC-MS Analysis and Gastroprotective Evaluations of Crude Extracts, Isolated Saponins, and Essential Oil from Polygonum hydropiper L.
GC-MS Analysis and Gastroprotective Evaluations of Crude Extracts, Isolated Saponins, and Essential Oil from Polygonum hydropiper L.
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水蓼粗提物、分离皂苷和精油的 GC-MS 分析和胃保护评价。
DOI：
10.3389/fchem.2017.00058
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发表时间：
2017
影响因子：
5.5
通讯作者：
Syed NI
中科院分区：
文献类型：
--
作者：
Ayaz M;Junaid M;Ullah F;Sadiq A;Shahid M;Ahmad W;Ullah I;Ahmad A;Syed NI
消化性溃疡是最常见的胃肠道疾病之一，其特征是胃蛋白酶和胃酸介导的粘膜损伤，是防御和进攻过程不平衡的结果。本研究采用结扎幽门大鼠模型，探讨水蓼粗甲醇提取物（Ph.Cr）对阿司匹林致溃疡的抗溃疡作用。使用标准方案评估体外脲酶和奇异变形杆菌抑制电位。所有馏分均采用气相色谱-质谱分析，以确定主要成分。阿司匹林致幽门结肠炎大鼠模型溃疡发生与溃疡平均评分[F(5,30) = 7.141, P = 0.0002]、胃液体积[F(5,30) = 8.245, P < 0.0001]、胃液pH [F(5,30) = 5.715, P = 0.0008]、游离酸度[F(5,30) = 4.544, P = 0.0033]、总酸度[F(5,30) = 2.740, P = 0.0373]、胃蛋白酶浓度[F(5,30) = 2.335, P = 0.0664]发生显著变化相关。溃疡生化指标显示，100mg /kg、200mg /kg和400mg /kg剂量的ph - cr预处理对阿司匹林诱导的幽门结扎溃疡模型具有明显的胃保护和抗溃疡作用。在脲酶抑制实验中，叶精油（Ph.Lo）、皂苷（Ph.Sp）和氯仿提取物（Ph.Chf）的抑制活性最高，IC50分别为90、98和520 μg/ml。Ph.Sp、Ph.Chf、Ph.EtAc和Ph.Cr对奇异芽孢杆菌的mic值分别为25、30、32.25和40.50 μg/ml。通过气相色谱-质谱分析鉴定出几种化合物。该植物溶剂提取物具有显著的体内抗溃疡、脲酶抑制和抗变形的潜力，表明其在消化性溃疡治疗中的潜在应用，并可能为该植物的传统用途提供科学依据。
Peptic ulceration is among the most prevalent gastrointestinal disorders characterized by pepsin and gastric acid mediated mucosal damage, as result of imbalance between defensive and offensive processes. The main objective of the current study was to investigate the antiulcer potentials of Polygonum hydropiper crude methanolic ectract (Ph.Cr) in aspirin induced ulcerogenesis using pylorus ligated rat model. In-vitro urease and Proteus mirabilis inhibitory potentials were evaluated using standard protocols. All fractions were analyzed using GC-MS to identify major components. The aspirin induced ulcerogenesis in pylorus ligated rat model was associated with significant changes in the mean ulcer score [F(5, 30) = 7.141, P = 0.0002], gastric juice volume [F(5, 30) = 8.245, P < 0.0001], gastric juice pH [F(5, 30) = 5.715, P = 0.0008], free acidity [F(5, 30) = 4.544, P = 0.0033], total acidity [F(5, 30) = 2.740, P = 0.0373], and pepsin concentration [F(5, 30) = 2.335, P = 0.0664]. Pre-treatment with Ph.Cr at 100, 200, and 400 mg/kg dose exhibited marked gastroprotective and anti-ulcerogenic effect in the aspirin induced pyloric ligation ulcerogenesis model at 100, 200, and 400 mg/kg as indicated by ulcerative biochemical parameters. In urease inhibition assay, leaves essential oil (Ph.Lo), saponins (Ph.Sp), and chloroform extract (Ph.Chf) exhibited highest activities with IC50 of 90, 98, and 520 μg/ml, respectively. Ph.Sp, Ph.Chf, ethyl acetate (Ph.EtAc), and Ph.Cr showed MICs of 25, 30, 32.25, and 40.50 μg/ml, respectively against P. mirabilis. Several compounds were identified in GC-MS analysis of samples. Significant in-vivo antiulcer, urease inhibitory as well as anti-proteus potentials of P. hydropiper solvent extracts, signify its potential use for the management of peptic ulcers and may provide scientific bases for the traditional uses of the plant.
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