Expression and function of the cdgD gene, encoding a CHASE-PAS-DGC-EAL domain protein, in Azospirillum brasilense.
Expression and function of the cdgD gene, encoding a CHASE-PAS-DGC-EAL domain protein, in Azospirillum brasilense.
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DOI：
10.1038/s41598-020-80125-3
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发表时间：
2021-01-12
影响因子：
4.6
通讯作者：
Ramírez-Mata A
中科院分区：
文献类型：
--
作者：
Cruz-Pérez JF;Lara-Oueilhe R;Marcos-Jiménez C;Cuatlayotl-Olarte R;Xiqui-Vázquez ML;Reyes-Carmona SR;Baca BE;Ramírez-Mata A
植物生长促进细菌巴西固氮螺菌含有多个编码参与第二信使环二-GMP生物合成和降解的蛋白质的基因，该蛋白质可能控制细菌的关键功能，例如生物膜形成和运动。在这里，我们分析了 cdgD 基因的功能和表达，该基因编码包含 GGDEF-EAL 结构域以及 CHASE 和 PAS 结构域的多结构域蛋白。构建了插入式cdgD基因突变体，对生物膜和细胞外聚合物产生以及运动表型的分析表明cdgD编码功能性二鸟苷酸蛋白。与野生型菌株中观察到的结果相比，这些结果与 48 小时内突变体中环二 GMP 的总体细胞浓度降低相关，而在补充菌株中则恢复了这一浓度。此外，在浮游或生物膜条件下生长的细胞中测量了cdgD基因表达，并且当向基本培养基中添加KNO3或NH4Cl作为氮源时观察到差异表达。 cdgD 启动子与编码自发荧光 mCherry 蛋白的基因的转录融合表明 cdgD 基因在非生物条件下以及与小麦根相关的情况下都表达。与在相同土壤条件下生长的野生型菌株相比，观察到突变体的小麦根定植减少。固氮螺菌与植物的关联始于细菌向植物根际的运动，随后这些细菌吸附并粘附到植物根部。因此，研究有助于细菌与其宿主植物初始相互作用的基因非常重要。
The plant growth-promoting bacterium Azospirillum brasilense contains several genes encoding proteins involved in the biosynthesis and degradation of the second messenger cyclic-di-GMP, which may control key bacterial functions, such as biofilm formation and motility. Here, we analysed the function and expression of the cdgD gene, encoding a multidomain protein that includes GGDEF-EAL domains and CHASE and PAS domains. An insertional cdgD gene mutant was constructed, and analysis of biofilm and extracellular polymeric substance production, as well as the motility phenotype indicated that cdgD encoded a functional diguanylate protein. These results were correlated with a reduced overall cellular concentration of cyclic-di-GMP in the mutant over 48 h compared with that observed in the wild-type strain, which was recovered in the complemented strain. In addition, cdgD gene expression was measured in cells growing under planktonic or biofilm conditions, and differential expression was observed when KNO3 or NH4Cl was added to the minimal medium as a nitrogen source. The transcriptional fusion of the cdgD promoter with the gene encoding the autofluorescent mCherry protein indicated that the cdgD gene was expressed both under abiotic conditions and in association with wheat roots. Reduced colonization of wheat roots was observed for the mutant compared with the wild-type strain grown in the same soil conditions. The Azospirillum-plant association begins with the motility of the bacterium towards the plant rhizosphere followed by the adsorption and adherence of these bacteria to plant roots. Therefore, it is important to study the genes that contribute to this initial interaction of the bacterium with its host plant.
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影响因子：
6.7
作者：
Jones CJ;Utada A;Davis KR;Thongsomboon W;Zamorano Sanchez D;Banakar V;Cegelski L;Wong GC;Yildiz FH
通讯作者：
Yildiz FH
二甘氨酸酸环化酶反应调节剂WSPR激活和自抑制的决定因素。
影响因子：
5.6
作者：
De, Nabanita;Navarro, Marcos V. A. S.;Raghavan, Rahul V.;Sondermann, Holger
通讯作者：
Sondermann, Holger
影响因子：
14.9
作者：
Finn RD;Clements J;Arndt W;Miller BL;Wheeler TJ;Schreiber F;Bateman A;Eddy SR
通讯作者：
Eddy SR
影响因子：
14.9
作者：
Letunic I;Doerks T;Bork P
通讯作者：
Bork P
DOI：
10.1007/978-1-4939-7240-1_12
发表时间：
2017-01-01
期刊：
C-DI-GMP SIGNALING
影响因子：
--
作者：
Jones, Christopher J.;Wozniak, Daniel J.
通讯作者：
Wozniak, Daniel J.