In vitro evaluation of anti-infective activity of a Lactobacillus plantarum strain against Salmonella enterica serovar Enteritidis.
In vitro evaluation of anti-infective activity of a Lactobacillus plantarum strain against Salmonella enterica serovar Enteritidis.
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DOI：
10.1186/1757-4749-5-11
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发表时间：
2013-05-13
期刊：
影响因子：
4.2
通讯作者：
Suar M
中科院分区：
文献类型：
--
作者：
Das JK;Mishra D;Ray P;Tripathy P;Beuria TK;Singh N;Suar M
已知肠道沙门氏菌肠炎血清型肠炎感染在世界范围内普遍存在，且发病率和死亡率增加。抗生素治疗和疫苗接种等传统策略不仅被证明疗效不佳，而且还导致沙门氏菌产生多重耐药菌株。近年来，益生菌针对各种肠道病原体的抗菌活性和其他健康促进作用变得更加重要。本研究旨在评估植物乳杆菌菌株（KSBT 56，从印度传统食品中分离出来）在体外预防肠炎沙门氏菌生长和致病性的功效。 KSBT 56菌株的无细胞培养上清液(CFCS)显着抑制肠炎沙门氏菌的生长，而不影响其他革兰氏阳性乳酸菌的生长。分离的 KSBT 56 菌株产生的乳酸与其他标准益生菌菌株（例如植物乳杆菌 MTCC 1407）相似。通过使用肠炎沙门氏菌的 sodC 突变体研究 KSBT 56 菌株产生的自由基，该突变体在 KSBT 56 菌株的 CFCS 存在下表现出生长减少，表明自由基对肠炎沙门氏菌生长的抑制活性。我们的结果还表明，在 KSBT 56 菌株存在下，肠炎沙门氏菌的生物膜形成能力显着降低（2 log cfu/ml，p = 0.01）。此外，通过庆大霉素保护试验验证了KSBT 56菌株的抗感染特性，结果显示肠炎沙门氏菌对HCT-116细胞系的侵袭减少了80%（肠炎沙门氏菌和KSBT 56的比例为1:1），并将肠炎沙门氏菌的添加延迟了1小时。同样，观察到沙门氏菌对 HCT-116 细胞的粘附力降低，同时沙门氏菌致病性岛 1 (SPI1) 的 hilA 基因下调，表明它们可能协同作用，减少病原体对细胞系的侵袭。 KSBT 56菌株有效抑制肠炎沙门氏菌的生长、侵袭和生物膜形成能力，且不抑制其他乳杆菌菌株的生长。总体而言，我们的结果表明 KSBT 56 可用作潜在的益生菌菌株，对宿主具有相当大的有益作用。
Salmonella enterica serovar Enteritidis infections are known to exhibit worldwide prevalence with increased morbidity and mortality. The conventional strategies like antibiotic therapy and vaccination have not only proved to be of sub-optimal efficacy but also led to the development of multidrug resistant strains of Salmonella. Antimicrobial activities of probiotics against various enteropathogens and other health promoting effects have assumed greater significance in recent years. The present study aims to evaluate the efficacy of a Lactobacillus plantarum strain (KSBT 56, isolated from a traditional food product of India), in preventing Salmonella enterica serovar Enteritidis growth and pathogenicity in vitro. The cell free culture supernatant (CFCS) of KSBT 56 strain notably inhibited the growth of Salmonella Enteritidis without affecting the growth of other gram-positive lactic acid bacteria. The isolated KSBT 56 strain produces lactic acid similar to other standard probiotic strains like Lactobacillus plantarum MTCC 1407. The free radical production by KSBT 56 strain was studied by using sodC mutant of S. Enteritidis, which exhibited reduced growth in the presence of CFCS of the KSBT 56 strain, indicating the inhibitory activity of free radicals on the growth of S. Enteritidis. Our results also showed a significant reduction in the biofilm forming ability of Salmonella Enteritidis in the presence of the KSBT 56 strain (2 log cfu/ml, p = 0.01). Further, the anti-infective characteristics of KSBT 56 strain was validated by gentamicin protection assay which revealed 80% reduction in the invasion of Salmonella Enteritidis to HCT-116 cell line (Salmonella Enteritidis and KSBT 56 in a 1:1 ratio) and delayed addition of Salmonella Enteritidis by 1 h. Similarly, the reduced adhesion of Salmonella to the HCT-116 cells was observed along with the down regulation of hilA gene of Salmonella Pathogenicity Island 1 (SPI1) indicating that they might have acted synergistically to decrease the invasion of the pathogen into the cell line. KSBT 56 strain effectively inhibited the growth, invasion and the biofilm forming ability of Salmonella Enteritidis without inhibiting the growth of other Lactobacillus strains. Overall, our result suggested that KSBT 56 can be used as a potential probiotic strain with considerable beneficial effects on the host.
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影响因子：
2.8
作者：
Botes, Marelize;Loos, Ben;Dicks, Leon M. T.
通讯作者：
Dicks, Leon M. T.
影响因子：
2.6
作者：
Soderling, Eva M.;Marttinen, Aino M.;Haukioja, Anna L.
通讯作者：
Haukioja, Anna L.
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
2
作者：
Bayoumi, Mohamed A.;Griffiths, Mansel W.
通讯作者：
Griffiths, Mansel W.
影响因子：
4.4
作者：
Moorthy, Guhapriya;Murali, Malliga Raman;Devaraj, S. Niranjali
通讯作者：
Devaraj, S. Niranjali