Potato calcium sensor modules StCBL3-StCIPK7 and StCBL3-StCIPK24 negatively regulate plant immunity.
Potato calcium sensor modules StCBL3-StCIPK7 and StCBL3-StCIPK24 negatively regulate plant immunity.
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马铃薯钙传感器模块StCBL3-StCIPK7和StCBL3-StCIPK24负向调节植物免疫。
DOI：
10.1186/s12870-023-04713-x
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发表时间：
2024-01-05
影响因子：
5.3
通讯作者：
Xu, Guangyuan
中科院分区：
文献类型：
--
作者：
Sun, Congcong;Li, Yuanyuan;Zhao, Tingting;Bi, Weishuai;Song, Yingying;Liang, Xiangxiu;Wang, Xiaodan;Dou, Daolong;Xu, Guangyuan
马铃薯晚疫病是由致病疫霉（Phytophthora infestans）引起的马铃薯主要病害。剖析马铃薯中的关键免疫组分将支持工程化致病疫霉抗性。在病原体入侵时，植物体内的Ca 2+信号由一系列Ca 2+传感器产生并解码，其中钙调神经磷酸酶B样蛋白（calcineurin B-like proteins，CBLs）与植物特异性CBL-interacting protein kinases（CIPKs）偶联在植物免疫中的研究较少。在这项研究中，我们确定了两个不同的马铃薯CBL-CIPK模块调节植物防御反应对疫霉和活性氧的产生，分别。通过部署基于病毒诱导的基因沉默（VIGS）系统的病原体接种测定，StCBL 3显示出负调节疫霉属抗性。同时，StCBL 3对本氏烟草的PTI和ETI反应具有负调控作用。此外，StCIPK 7被鉴定为与StCBL 3一起作用以负调节疫霉属抗性。StCIPK 7与StCBL 3物理相互作用并以Ca 2+依赖性方式磷酸化StCBL 3。StCBL 3促进StCIPK 7激酶活性。另一方面，另一种StCBL 3相互作用激酶StCIPK 24通过与StRBOHB相互作用负调节flg 22触发活性氧（ROS）的积累。总之，这些发现表明StCBL 3-StCIPK 7复合物负向调节疫霉抗性，StCBL 3-StCIPK 24复合物负向调节活性氧的产生。本研究结果为马铃薯CBL-CIPK在植物免疫中的作用提供了新的认识，为马铃薯抗病基因工程提供了宝贵的基因资源。在线版本包含补充材料，可通过10.1186/s12870-023-04713-x获得。
Potato late blight, caused by Phytophthora infestans, is the most devastating disease on potato. Dissecting critical immune components in potato will be supportive for engineering P. infestans resistance. Upon pathogens attack, plant Ca2+ signature is generated and decoded by an array of Ca2+ sensors, among which calcineurin B-like proteins (CBLs) coupled with plant specific CBL-interacting protein kinases (CIPKs) are much less explored in plant immunity. In this study, we identified that two differential potato CBL-CIPK modules regulate plant defense responses against Phytophthora and ROS production, respectively. By deploying virus-induced gene silencing (VIGS) system-based pathogen inoculation assays, StCBL3 was shown to negatively regulate Phytophthora resistance. Consistently, StCBL3 was further found to negatively regulate PTI and ETI responses in Nicotiana benthamiana. Furthermore, StCIPK7 was identified to act together with StCBL3 to negatively regulate Phytophthora resistance. StCIPK7 physically interacts with StCBL3 and phosphorylates StCBL3 in a Ca2+-dependent manner. StCBL3 promotes StCIPK7 kinase activity. On the other hand, another StCBL3-interacting kinase StCIPK24 negatively modulating flg22-triggered accumulation of reactive oxygen species (ROS) by interacting with StRBOHB. Together, these findings demonstrate that the StCBL3-StCIPK7 complex negatively modulates Phytophthora resistance and StCBL3-StCIPK24 complex negatively regulate ROS production. Our results offer new insights into the roles of potato CBL-CIPK in plant immunity and provide valuable gene resources to engineer the disease resistance potato in the future. The online version contains supplementary material available at 10.1186/s12870-023-04713-x.
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