Multilocus Sequence Types of Campylobacter jejuni Isolates from Different Sources in Eastern China

Multilocus Sequence Types of Campylobacter jejuni Isolates from Different Sources in Eastern China
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中国东部地区不同来源空肠弯曲菌的多位点序列类型

DOI:
10.1007/s00284-015-0853-3
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发表时间:
2015-06
影响因子:
2.6
通讯作者:
Huang Jinlin
Huang Jinlin
中科院分区:
生物学4区
文献类型:
--
作者:
Zhang Gong;Zhang Xiaoyan;Hu Yuanqing;Jiao Xin-an;Huang Jinlin

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摘要空肠弯曲菌是引起人类胃肠炎的主要食源性致病菌,在许多发达国家都存在。在我们的研究中,我们将多位点序列分型(MLST)技术应用于167 C。从中国东部不同来源的空肠分离株,以研究其遗传多样性。MLST定义了18个克隆复合体(CC)的94个序列类型(ST)。 来自60个分离株(36%)的45个ST和22个等位基因先前未在国际数据库中记录。102个菌株分属于8个克隆复合体,占总菌株数的61.1%.八个主要的CC也是来自不同来源的最常见的复合物。从人类和食物中分离的ST型菌株最常见的是ST-353。在鸡和食品中占优势的ST类型是ST-354。在含有两种或两种以上不同来源分离株的21种ST中,15种ST含有人类分离株和来自其他来源的分离株,这表明潜在致病性菌株并不限于特定谱系。
AbstractCampylobacter jejuni is a major food-borne pathogen that causes human gastroenteritis in many developed countries. In our study, we applied multilocus sequence typing (MLST) technology to 167 C. jejuni isolates from diverse sources in Eastern China to examine their genetic diversity. MLST defined 94 sequence types (STs) belonging to 18 clonal complexes (CCs). Forty-five STs from 60 isolates (36 %) and 22 alleles have not been previously documented in an international database. One hundred and two isolates, accounting for 61.1 % of all isolates, belonged to eight clonal complexes. The eight major CCs were also the most common complexes from different sources. The most common ST type of isolates from human and food was ST-353. The dominant ST type in chicken and foods was ST-354. Among 21 STs that contained two or more different sources isolates, 15 STs contained human isolates and isolates from other sources, suggesting that potentially pathogenic strains are not restricted to specific lineages.
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