The polarity of the proximal tubule cell in rat kidney. Different surface charges for the brush-border microvilli and plasma membranes from the basal infoldings.
The polarity of the proximal tubule cell in rat kidney. Different surface charges for the brush-border microvilli and plasma membranes from the basal infoldings.
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DOI：
10.1083/jcb.54.2.232
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发表时间：
1972-08
期刊：
影响因子：
--
通讯作者：
Hannig K
中科院分区：
文献类型：
--
作者：
Heidrich HG;Kinne R;Kinne-Saffran E;Hannig K
在制备自由流电泳法中，从大鼠肾皮质刷状缘部位利用不同的表面电荷获得了两种不同的膜部位。一种膜组分只含有形态完整的微绒毛，具有较高的碱性磷酸酶比活性。另一组分的形态与经典质膜相似，具有连接复合体和较高的Na-K-ATPase活性，分离的膜组分被其他细胞器污染极低，这两个组分代表了肾近端小管细胞膜的顶端(腔)和基底区(间质)，清楚地显示了该细胞的极性。
Two different membrane fractions were obtained from a brush-border fraction of rat kidney cortex by using their different electrical surface charges in preparative free-flow electrophoresis. One membrane fraction contained only morphologically intact microvilli and was characterized by a high specific activity of alkaline phosphatase. The other fraction morphologically resembled classical plasma membranes by possessing junctional complexes and a high Na-K-ATPase activity The contamination of the isolated membrane fractions by other cell organelles was extremely low These two fractions represent the apical (luminal) and the basal (interstitial) area of the renal proximal tubule cell membrane and clearly demonstrate the polarity of this cell.
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影响因子：
56.9
作者：
BINKLEY, F;KING, N;WUNDRAM, IJ
通讯作者：
WUNDRAM, IJ
影响因子：
7.8
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Baudhuin, P;Beaufay, H;De Duve, C
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STAHN, R;MAIER, KP;HANNIG, K
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4.5
作者：
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通讯作者：
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影响因子：
4.5
作者：
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通讯作者：
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