Efficacy and immunogenicity of recombinant swinepox virus expressing the A epitope of the TGEV S protein.
Efficacy and immunogenicity of recombinant swinepox virus expressing the A epitope of the TGEV S protein.
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DOI：
10.1016/j.vaccine.2015.06.057
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发表时间：
2015-07-31
期刊：
影响因子：
5.5
通讯作者：
Fan H
中科院分区：
文献类型：
--
作者：
Yuan X;Lin H;Fan H
为探索研制猪传染性胃肠炎病毒（transmissible gastroenteritis virus，TGEV）疫苗的可能性，构建了表达TGEV保护性抗原的重组猪痘病毒（rSPV-SA）。在小鼠和猪模型中评估疫苗接种载体的免疫应答和保护效力。间接ELISA检测结果表明，rSPV-SA免疫小鼠后，小鼠血清中抗TGEV的IgG水平显著提高。结果表明，rSPV-SA免疫小鼠后，Th 1型和Th 2型细胞因子水平均升高。新生仔猪被动免疫保护试验结果表明，重组SPV-SA活载体疫苗能100%保护仔猪免受SPV感染，且在本试验期间，rSPV-SA治疗组未出现明显的临床症状。这些数据表明，新的重组猪痘病毒是一种潜在的疫苗，对TGEV感染。
To explore the possibility of developing a vaccine against transmissible gastroenteritis virus (TGEV) infection, a recombinant swinepox virus (rSPV-SA) expressing a TGEV protective antigen has been constructed. Immune responses and protection efficacy of the vaccination vector were assessed in both mice and pig models. An indirect ELISA assay suggested that when mice were vaccinated with rSPV-SA, the level of IgG against TGEV was enhanced dramatically. The cytokine assays were employed and the results indicated that both the Th1-type and Th2-type cytokine levels raised after vaccination with rSPV-SA in mice models. Results from the passive immunity protection test of new born piglets demonstrated that the recombinant live-vector vaccine, rSPV-SA, could 100% protect piglets from the SPV infection, and there was no significant clinical symptom in the rSPV-SA treatment group during this experiment. The data suggest that the novel recombinant swinepox virus is a potential vaccine against TGEV infection.
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影响因子：
5
作者：
Xu, Jiarong;Huang, Dongyan;Lu, Chengping
通讯作者：
Lu, Chengping
通过免疫带有 TGEV S1 基因的 DNA 质粒产生的抗传染性胃肠炎病毒的单克隆抗体
DOI：
10.1089/mab.2012.0067
发表时间：
2013-02-01
影响因子：
--
作者：
Zhao, Qiong;Zhu, Jiayi;Ren, Xiaofeng
通讯作者：
Ren, Xiaofeng
影响因子：
1.6
作者：
Zheng Q;Chen D;Li P;Bi Z;Cao R;Zhou B;Chen P
通讯作者：
Chen P
影响因子：
5.5
作者：
TAYLOR, J;WEINBERG, R;PAOLETTI, E
通讯作者：
PAOLETTI, E
影响因子：
3.1
作者：
Hahn, J;Park, SH;Ahn, BY
通讯作者：
Ahn, BY